Mouse E14 sectioned microCT Movie

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Mouse embryo E14 sectioned microCT icon.jpg

Mouse embryo, Theiler Stage 22

  • Fingers separate distally
  • Individual 'fingers' are visible in the anterior footplate and there are deep indentations between the 'toes' which are not yet separated.
  • The long bones of the limbs are present and there are hair follicles in the pectoral, pelvic and trunk regions.
  • The pinna is turned forwards and the umbilical hernia is conspicuous.
  • Absent: hair follicles in the cephalic region.
  • Embryonic age = 14 dpc (range 13.5-15 dpc)
  • 56-60 somite pairs
  • Equivalent Witschi Stage in rat = 31
  • Equivalent Carnegie Stage in humans = 20-23


Links: MP4 version | Quicktime version 2.2MB | Quicktime version 4MB | Movies | Computed Tomography | Mouse Development

Reference

Brian D Metscher MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues. BMC Physiol.: 2009, 9;11 PubMed 19545439

| PMC2717911 | BMC Physiol.

Copyright

© 2009 Metscher; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


IKI stain - 1% iodine metal (I2) + 2% potassium iodide (KI) in water.