File:Telencephalon development signals.jpg

Telencephalon Development

Changes in the regionalized expression of downstream effectors of Fgfs and of Wnts molecules in the absence of defects at signaling centers.

(A–H) E12.5 coronal sections of control (A, C, E, and G) and Dbx1DTA;Emx1iresCre (B, D, F, H) telencephalons were hybridized with Erm (A and B), Pea3 (C and D), Wnt7b (E and F), Wnt8b (G and H) RNA probes (n = 3).

In mutant animals, Erm expression is reduced in DM/D and unaltered in ventromedial regions (compare [A] and [B]).

Pea3 expression in the DM/D domain is decreased in mutant embryos (compare [C] and [D]).

Wnt7b expression domain is extended ventrally in the medial pallium and its expression intensity is increased in mutant embryos (compare [E] and [F]).

An increase in the size and intensity of the Wnt8b expression domain is detected in mutant animals in DM region (H) compared to controls in which, at this level, Wnt8b is barely detectable (G).

White and red arrowheads indicate limits of high expression domains in controls and mutant animals, respectively. (I–L) In situ hybridization with Wnt8b RNA probe on E11.5 control (I and K) and Dbx1DTA;Emx1iresCre (J and L) embryos on two serial rostral coronal sections.

The Wnt8b expression domain is expanded rostrally and ventrally in Dbx1DTA;Emx1iresCre embryos (J and L), compared to control (I and K) embryos. Black arrowheads delimit the Wnt8b expression domain in control, and red arrowheads the ventral limit of shifted domains in mutant embryos (n = 3). (M–T) In situ hybridization on E11.5 sections of control (M, O, Q, and S) and Dbx1DTA;Emx1iresCre embryos (N, P, R, and T) (n = 4).

No difference is observed between control and mutant embryos for the expression of Fgf17 (M and N), Fgf8 (O and P), Fgf15 (Q and R) and Wnt3a (S and T).

Scale bars: 200 µm (A–T).

Original file name: Figure 4. doi:10.1371/journal.pbio.1000440.g004 http://www.plosbiology.org/article/slideshow.action?uri=info:doi/10.1371/journal.pbio.1000440&imageURI=info:doi/10.1371/journal.pbio.1000440.g004

Reference

<pubmed>20668538</pubmed>| PLoS Biol.

Copyright: © 2010 Griveau et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.