File:Rat-hindbrain E11.5.jpg

Double in situ staining of mRNAs for down-regulated genes and Pax6 in the E11.5 rat hindbrain.

The expression sites of Dbx1, Unc5h1 and Cyp26b1 (blue-violet in A-I and red in J-L) were compared with that of Pax6 (pink in A-I and blue in J-L) in WT (A, D, G, J), rSey2/+ (B, E, H, K) and rSey2/rSey2 (C, F, I, L) hindbrains in 'open-book' preparations.

(A-C) Dbx1 expression is decreased throughout the interneuron progenitor domain of the rSey2/rSey2 hindbrain with the exception of r1, where Pax6 is not expressed (asterisk in C).

(D-F) Unc5h1 expression disappears in the somatic motor neuron progenitor domain and in the dorsal region of r7 in the rSey2/rSey2 hindbrain (F).

(G-L) Cyp26b1 expression is specifically altered in the dorsolateral region of r5 and r6 in the rSey2/rSey2 hindbrain (arrowheads in I and L). r1 to r7 indicate the individual rhombomeres of the hindbrain.

Scale bar: 500 μm.

1471-213X-10-6-5.jpg

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2818624/?tool=pmcentrez

BMC Dev Biol. 2010; 10: 6.

Published online 2010 January 18. doi: 10.1186/1471-213X-10-6.

Copyright ©2010 Numayama-Tsuruta et al; licensee BioMed Central Ltd.

Downstream genes of Pax6 revealed by comprehensive transcriptome profiling in the developing rat hindbrain. Numayama-Tsuruta K, Arai Y, Takahashi M, Sasaki-Hoshino M, Funatsu N, Nakamura S, Osumi N. BMC Dev Biol. 2010 Jan 18;10:6. PMID: 20082710

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.