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Plate 2

FIG. 3. Ovarian egg from a doe mated 4 hours previously. Tetrads fully formed and vesicular nucleus dissolved.

FIG. 4. Ovarian egg from a doe mated 5 hours previously. The tetrads have become smaller and arranged themselves in a plate. All traces of the vesicular membrane have disappeared.

FIG. 7. Ovarian egg from a doe mated 9 hours previously. First polar body and second polar spindle.

FIG. 8. Ovarian egg cultured for 24 hours in Ringer-Locke solution containing maturity hormone. Note apparent fusion nuclei.

FIG. 11. Ovarian egg from a doe mated 6 hours previously and inseminated in vitro with normal sperm. Male and female pronuclei present side by side.

FIG. 12. Ovarian egg from a doe mated 8 hours previously and inseminated with normal sperm. The first polar body has formed and sperm penetration occurred. The entering spermatozoan has formed a male pronucleus (centre left).

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