File:Patterns of ZPC Deposition in Porcine Oocytes.jpg

Patterns of ZPC Deposition in Porcine Oocyte-Cumulus Complexes

The zona pellucida protein, ZPC, is a receptor for the spermatozoon in fertilisation.

Immunofluorescence detection for ZPC and ubiquitin in a porcine oocyte.

(a, b) ZPC (grey) detected in ridges (arrowheads) adjacent to zona-adhering corona radiata cells (nuclei stained blue).

(c-e) ZPC (red) and ubiquitin (green) detected in the ZP.

(c, d) ZPC (red) and ubiquitin (green) in the cytoplasm of corona radiata cells.

(e) DNA for ZPC counterstained with DAPI (blue).

(f) Western blotting of ZPC protein in the isolated cumulus/corona cells from 60 oocyte cumulus complexes (lane 1), in 60 zona-free oocytes (lane 2) and in soluble zona proteins isolated from 60 oocytes (lane 3).

References

Zimmerman SW, Manandhar G, Yi YJ, Gupta SK, Sutovsky M, Odhiambo JF, Powell MD, Miller DJ & Sutovsky P. (2011). Sperm proteasomes degrade sperm receptor on the egg zona pellucida during mammalian fertilization. PLoS ONE , 6, e17256. PMID: 21383844 DOI.

Copyright

© 2011 Zimmerman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Original file name: Figure 6. doi:10.1371/journal.pone.0017256.g006 http://www.plosone.org/article/info:doi/10.1371/journal.pone.0017256#pone-0017256-g006

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