File:Mouse zygote paternal genome reprogramming 01.jpg
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Mouse Zygote Paternal Genome Reprogramming
Mouse zygotes male pronucleus contains 5-hydroxymethylcytosine (5hmC) formed by enzymatic oxidation of 5-methylcytosine (5mC).
- A - A mouse zygote was double-stained with anti-5hmC antibody (green) and anti-5mC antibody (red). The smaller maternal pronucleus is closer to the polar body (pb). A bright-field image is shown on the far left.
- B - Additional zygotes were double-stained with anti-5hmC antibody (green) and anti-5mC antibody (red). Merged images are shown.
- C - Zygotes obtained by in vitro fertilization were double-stained similarly. Two polyspermic zygotes (to the right) exhibit 5hmC staining in two paternal pronuclei.
- D - 5mC and 5hmC staining reveal two separate chromosome sets at metaphase of zygote division. A confocal image is shown.
- E - Individual chromosomes are largely stained for either 5mC (likely originated from the maternal pronucleus) or 5hmC (likely from the paternal pronucleus) at anaphase of zygote division. Two Z sections of the same zygote are shown.
- 5hmC - 5-hydroxymethylcytosine (formed by enzymatic oxidation of 5mC).
- 5mC - 5-methylcytosine.
- Links: Epigenetics | Zygote | Mouse Development
Reference
<pubmed>21321204</pubmed>| PMC2132672 | PNAS
Copyright
Proceedings National Academy of Sciences (PNAS) Liberalization of PNAS copyright policy: Noncommercial use freely allowed Note original Author should be contacted for permission to reuse for Educational purposes. See also PNAS Author Rights and Permission FAQs
- Cozzarelli NR, Fulton KR, Sullenberger DM. Liberalization of PNAS copyright policy: noncommercial use freely allowed. Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12399. PMID15314225 "Our guiding principle is that, while PNAS retains copyright, anyone can make noncommercial use of work in PNAS without asking our permission, provided that the original source is cited."
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048122/figure/fig01/
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 08:01, 16 June 2012 | | 1,157 × 1,280 (178 KB) | Z8600021 (talk | contribs) | ==Mouse Zygote Paternal Genome Reprogramming== 5-hydroxymethylcytosine (5hmC), most likely derived from enzymatic oxidation of 5-methylcytosine (5mC) 5hmC is present in the male pronucleus of mouse zygotes. (A) A mouse zygote was double-stained with an |
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