File:Mouse antral follicle 01.jpg

FSH antral follicles: Oocyte and Granulosa cells

Eighty (80)% of FSH-treated follicles formed the antral cavity (FSH antral follicles). These follicles showed various ultrastructural markers of maturity (spreading of organelles in ooplasm, abundant microvilli on the oolemma, scarce granulosa cell-oolemma contacts, granulosa cell proliferation).

The oocytes (O) contain numerous organelles, uniformly distributed in the ooplasm (panels a, b, d). Note the presence of scattered cortical granules (arrows) in the suboolemmal area (panel b). Numerous microvilli (m) can be observed on the oocyte surface (panels b, c). Cytoplasmic projections (arrows) stemming from the inner granulosa cell (GC) layer are seen crossing the zona pellucida (ZP) (panels a, d) and reaching the oocyte (transzonal processes) (panels d, e). Areas of detachment between inner granulosa cells and oocyte are also present (asterisks, panels a, d). Outer granulosa cells (GC) appeared irregularly rounded/polygonal and scarcely adherent each other (panels f, h). Uniformly dispersed chromatin and one or more nucleoli are seen in both inner (panels a, d) and outer (panels f, g) granulosa cells. Panel b, ZP: zona pellucida. Panel e, O: oocyte. Panel f, arrow: loss of contact among granulosa cells; panel g, L: lipid droplets in the granulosa cell cytoplasm.

Bar is: 3.5 μm (panel a); 1 μm (panel b); 10 μm (panel c); 2 μm (panel d); 9 μm (panel e); 3 μm (panels f, g); 50 μm (panel h). Panels a, b, d, f, g: TEM; panels c, e, h: SEM.

Original file name: 1477-7827-9-3-4-l.jpg

Reference

<pubmed>21232101</pubmed>| PMC3033320 | Reprod Biol Endocrinol.

Nottola et al. Reproductive Biology and Endocrinology 2011 9:3 doi:10.1186/1477-7827-9-3

© 2011 Nottola et al; licensee BioMed Central Ltd.

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