File:Mouse CT E10.5 head.jpg

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Mouse Embryo Computed Tomography

Identically scaled renderings representing 3D μCT scans of embryos (E10.5 head according to paper methods) subjected to different fixation and scanning procedures.

A - 4% formaldehyde and secondary Bouin's fixation.
B - 4% formaldehyde + 1% glutaraldehyde with Iothalamate meglumine used as a contrast agent.
C - 4% formaldehyde + 5% glutaraldehyde plus contrast agent.

All embryos are shown in right 3/4 inferolateral view.

"Typical μCT renderings are represented by the embryos depicted in Figure 2A-C. The combination of aldehyde fixation and Cysto Conray® yields images (Figure 2B-C) with a surface quality comparable to embryos fixed with Bouin's solution (Figure 2A). Bouin's-fixed embryos appear have a rougher surface texture compared to those fixed with either glutaraldehyde mixture (Figure 2B-C). The identically scaled images of Bouin's fixed embryos appeared generally smaller compared to those fixed with glutaraldehyde mixtures, but a comparison of μCT images only cannot indicate the relative roles of fixation-induced tissue shrinkage or swelling."


Mouse Stages: E1 | E2.5 | E3.0 | E3.5 | E4.5 | E5.0 | E5.5 | E6.0 | E7.0 | E7.5 | E8.0 | E8.5 | E9.0 | E9.5 | E10 | E10.5 | E11 | E11.5 | E12 | E12.5 | E13 | E13.5 | E14 | E14.5 | E15 | E15.5 | E16 | E16.5 | E17 | E18.5 | E18 | E18.5 | E19 | E20 | Timeline | About timed pregnancy


Species Embryonic Comparison Timeline
Carnegie Stage
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
Human Days 1 2-3 4-5 5-6 7-12 13-15 15-17 17-19 20 22 24 28 30 33 36 40 42 44 48 52 54 55 58
Mouse Days 1 2 3 E4.5 E5.0 E6.0 E7.0 E8.0 E9.0 E9.5 E10 E10.5 E11 E11.5 E12 E12.5 E13 E13.5 E14 E14.5 E15 E15.5 E16
Rat Days 1 3.5 4-5 5 6 7.5 8.5 9 10.5 11 11.5 12 12.5 13 13.5 14 14.5 15 15.5 16 16.5 17 17.5
Note these Carnegie stages are only approximate day timings for average of embryos. Links: Carnegie Stage Comparison
Table References  
Human

O'Rahilly R. (1979). Early human development and the chief sources of information on staged human embryos. Eur. J. Obstet. Gynecol. Reprod. Biol. , 9, 273-80. PMID: 400868
Otis EM and Brent R. Equivalent ages in mouse and human embryos. (1954) Anat Rec. 120(1):33-63. PMID 13207763

Mouse
Theiler K. The House Mouse: Atlas of Mouse Development (1972, 1989) Springer-Verlag, NY. Online
OTIS EM & BRENT R. (1954). Equivalent ages in mouse and human embryos. Anat. Rec. , 120, 33-63. PMID: 13207763

Rat
Witschi E. Rat Development. In: Growth Including Reproduction and Morphological Development. (1962) Altman PL. and Dittmer DS. ed. Fed. Am. Soc. Exp. Biol., Washington DC, pp. 304-314.
Pérez-Cano FJ, Franch À, Castellote C & Castell M. (2012). The suckling rat as a model for immunonutrition studies in early life. Clin. Dev. Immunol. , 2012, 537310. PMID: 22899949 DOI.

Timeline Links: human timeline | mouse timeline | mouse detailed timeline | chicken timeline | rat timeline | Medaka | Category:Timeline

Reference

<pubmed>20163731</pubmed>| BMC Developmental Biology


© 2010 Schmidt et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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current11:24, 17 August 2010Thumbnail for version as of 11:24, 17 August 20101,200 × 449 (83 KB)S8600021 (talk | contribs)==Mouse Embryo Computed Tomography== Identically scaled renderings representing 3D μCT scans of embryos (E10.5 head according to paper methods) subjected to different fixation and scanning procedures. A, 4% formaldehyde and secondary Bouin's fixation.
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