File:Mouse Blastocysts stained with different immunoflurosecent markers.jpg

PMID: 20485553

Expression and localization of Cdx2 and Oct4 in Brg1 KD blastocysts.

(A) ICC analysis of Oct4 and Cdx2 in Brg1 KD and control blastocysts. In control blastocysts (a–d) Oct4 expression (green) is restricted to the ICM and is largely absent in the Cdx2-positive (red) trophectoderm. In contrast, in Brg1 KD blastocysts (e–h) Oct4 is widely expressed in both the ICM and cdx2-positive (yellow) trophectoderm. Arrowheads denote co-expression of Oct4 and Cdx2. Nuclei were counter stained with DAPI (blue). (B) Quantification of the average number of cells in Brg1 KD and control blastocysts expressing Oct4, Cdx2, and Oct4 & Cdx2 (double expression). Asterisks denote statistical significance (p<0.05) between Brg1 KD and control blastocysts. A total of 25 Brg1 KD blastocysts and 15 control blastocysts were analyzed.

http://dx.doi.org/10.1371/journal.pone.0010622.g001

Reference

<pubmed>20485553</pubmed>

Wang K, Sengupta S, Magnani L, Wilson CA, Henry RW, Knott JG. Brg1 Is Required for Cdx2-Mediated Repression of Oct4 Expression in Mouse Blastocysts. Dey SK, ed. PLoS ONE. 2010;5(5):e10622. doi:10.1371/journal.pone.0010622.

Copyright

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