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Mouse - Analysis of Colonic Microbiota

Following total colonic DNA extraction, PCR was performed with 8 bar coded Bact27 and UNIV788 primers to amplify the bacterial 16S rRNA gene. PCR products were purified and underwent subsequent sequencing on the Roche GS-FLX pyrosequencing platform. Taxonomy was assigned to sequences using the Ribosomal Database Project classifier tool.

Mice with NEC (a) are compared to mice without NEC (b).

Statistical tests for differentially abundant 16S-based taxonomic annotations between populations were performed using Metastats methodology to compute nonparametric p-values. Differences were considered significant at p<0.05.

* indicates statistically significantly more in mice with NEC.

** indicates statistically significantly more in mice without NEC.

Original file name: Figure 5. Journal.pone.0018084.g005.png

doi:10.1371/journal.pone.0018084.g005

Reference

<pubmed>21445365</pubmed>| PLoS One.


Copyright: © 2011 Carlisle et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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current16:26, 2 May 2011Thumbnail for version as of 16:26, 2 May 2011600 × 422 (47 KB)S8600021 (talk | contribs)==Mouse - Analysis of Colonic Microbiota== Following total colonic DNA extraction, PCR was performed with 8 bar coded Bact27 and UNIV788 primers to amplify the bacterial 16S rRNA gene. PCR products were purified and underwent subsequent sequencing on the
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