File:In vitro kidney development.png

Kidneys grown on glass develop organotypic features including cortico-medially zonation

(A) Shows a time course of development to 240 h (10 d). From about 108 h (4.5 d), the kidney spreads out enough that it begins to divide into two zones, an outer medullary zone that features many nephrons and an inner medulla that contains more elongated tubules (mainly collecting ducts at this stage). By 240 h (10 d), this effect has become more marked. The last panel of (A) shows a kidney rudiment cultured on a filter for 240 h: the organ occupies less area and there is less evidence of corticomedullary zonation. The ‘grain’ in the photograph is an optical effect of the filter pores, which are beneath the kidney; their absence in bright field imaging is another advantage of the glass system.

(B) A high-power view of part of a 240 h kidney shows that nephrons (red) are arranged organotypically, with the glomerulus (‘Glom’) and proximal convoluted tubule (‘PCT’) in the cortex and a loop of Henle (‘LoH’) extending down into the medulla, forming a tight hair-slide shape parallel to the collecting ducts (green). Green = calbindin, red = laminin.

Original File Name: Figure 6. Journal.pone.0010550.g006.png http://www.plosone.org/article/slideshow.action?uri=info:doi/10.1371/journal.pone.0010550&imageURI=info:doi/10.1371/journal.pone.0010550.g006

Reference

<pubmed>20479933</pubmed>| PLoS One.

Copyright: © 2010 Sebinger et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.