File:Human cytomegalovirus beta-catenin juxtanuclear region.jpg
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Human Cytomegalovirus infection inhibits β-catenin nuclear translocation in EVTs
HCMV infection leads to β-catenin protein accumulation in a discrete juxtanuclear region.
- Extravillous trophoblast (EVT) cell line (SGHPL-4) derived from first trimester placenta.
- Cells were seeded on glass coverslips and infected with Towne-GFP (MOI of 1–2) or mock-infected for 48 hr.
- Cells were then treated with 150 ng/ml Wnt-3A or 20 mM LiCl for 6 hr followed by immunostaining for β-catenin using a mouse anti-β catenin antibody, followed by a goat anti-mouse secondary IgG conjugated to AlexaFluor 555.
- Nuclei were counterstained with DAPI. Mouse IgG was used as an isotype control. GFP positive cells represent infected cells. Arrowheads point to nuclear accumulation of β-catenin. Arrows indicate aggregation of β-catenin in infected cells.
Reference
Angelova M, Zwezdaryk K, Ferris M, Shan B, Morris CA & Sullivan DE. (2012). Human cytomegalovirus infection dysregulates the canonical Wnt/β-catenin signaling pathway. PLoS Pathog. , 8, e1002959. PMID: 23071438 DOI.
HCMV image panels cropped from Figure 5. HCMV infection inhibits β-catenin nuclear translocation in EVTs. Journal.ppat.1002959.g005.jpg
Copyright
© Angelova et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Cite this page: Hill, M.A. (2024, April 24) Embryology Human cytomegalovirus beta-catenin juxtanuclear region.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Human_cytomegalovirus_beta-catenin_juxtanuclear_region.jpg
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
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