File:Human Carnegie stage 13 stem cell markers.jpg

Pluripotent stem cell markers are expressed by uncommitted hNCC.

(B) Expression of NANOG mRNA in transverse section of human Carnegie stage 13 (C13) embryo (cf. Fig. 4H) is discrete but present in neural tube, neural crest cells in the dorsal root (arrowhead) and dermatome, when compared with a sense probe hybridized adjacent section (C).

(D) Expression of POU5F1 in simultaneously hybridized adjacent section is more visible in equivalent structures, and seems to have a widespread basal expression in all tissues.

(E) NANOG expression at C15 (cf. Fig. 9F and G). After a few days’ growth, expression is more distinct in the proliferating neuroepithelium, dorsal root ganglia, the motor horns, stomach, sympathetic ganglia, liver bud and migrating myotome cells.

(F) Adjacent section hybridized with sense probe.

(G) POU5F1 is expressed in a similar pattern. Both NANOG and POU5F1 transcripts appear to be excluded from the roofplate of the neural tube at this stage (extension of dotted lines).

Abbreviations: da, dorsal aorta; dm, dermamyotome; drg, dorsal root ganglion; es, stomach; lv, liver; mn, mesonephros; nt, neural tube; sg, sympathetic ganglion.

Extracted from Figure 6. http://hmg.oxfordjournals.org/content/vol17/issue21/images/large/ddn23506.jpeg

Links: Carnegie stage 13

Human neural crest cells display molecular and phenotypic hallmarks of stem cells. Thomas S, Thomas M, Wincker P, Babarit C, Xu P, Speer MC, Munnich A, Lyonnet S, Vekemans M, Etchevers HC. Hum Mol Genet. 2008 Nov 1;17(21):3411-25. PMID: 18689800 | Hum Mol Genet.

© 2008 The Author(s)

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