File:Extracellular calcium levels compared to Spermatozoa levels.jpeg
Extracellular_calcium_levels_compared_to_Spermatozoa_levels.jpeg (600 × 391 pixels, file size: 17 KB, MIME type: image/jpeg)
Effect of extracellular Ca2+ depletion on the human zona pellucida-induced intracellular [Ca2+] increase in spermatozoa
Capacitated, Fura-2-loaded spermatozoa were incubated in normal BWW medium (upper curve) or in BWW medium lacking calcium (lower curve). One minute before adding hZP, EGTA (500 μM) was added to the Ca2+ free BWW, to ensure that the extracellular medium was devoid of Ca2+. Solubilised hZP (2/μl) was added (arrow) to the medium for each incubation. Results are expressed as the fluorescence excitation ratio (340/380 excitation wavelengths, in arbitrary fluorescence units (AFU)) as a function of time (min). A typical experiment is shown.
Reference
<pubmed> PMC1712232 </pubmed>
Copyright
Copyright © 2006 Patrat et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 19:06, 2 September 2016 | | 600 × 391 (17 KB) | Z5015337 (talk | contribs) | Needed to rename the file to use the properly titled picture. |
| 09:57, 19 August 2016 |  | 600 × 391 (17 KB) | Z5015337 (talk | contribs) | Effect of extracellular Ca2+ depletion on the human zona pellucida-induced intracellular [Ca2+] increase in spermatozoa. Capacitated, Fura-2-loaded spermatozoa were incubated in normal BWW medium (upper curve) or in BWW medium lacking calcium (lower cu... | |
| 09:52, 19 August 2016 |  | 600 × 391 (17 KB) | Z5015337 (talk | contribs) | Added Extracellular calcium levels compared to Spermatozoa levels.jpg |
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