File:Cord blood induced stem cells 01.jpg
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Generation of non-viral induced pluripotent stem cells (hiPSC) from CD34+ cord blood progenitors
(A) Representative immunocytochemistry of pluripotency markers POU5F1 (OCT4), NANOG, TRA-1-81, and SSEA4 in hiPSC line CBiPSC6.2 after >20 passages.
(B) Representative hematoxylin and eosin staining of teratoma sections derived from CBiPSC6.2 after >20 passages demonstrating ectodermal, endodermal and mesodermal lineage differentiation. All CBiPSC clones in these studies formed similar teratomas.
(C) Real-time RT-PCR studies p15 CBiPSC lines for endogenous pluripotency genes using primers that distinguish endogenous expression from transgenes (see Methods).
Original image name: Figure 2. doi:10.1371/journal.pone.0018293.g002 (cropped A-C from full figure)
Reference
<pubmed>21494607</pubmed>| PMC3072973 | PLoS One.
Copyright: © 2011 Burridge et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 12:42, 12 October 2011 | | 800 × 787 (157 KB) | S8600021 (talk | contribs) | ==Generation of non-viral induced pluripotent stem cells (hiPSC) from CD34+ cord blood progenitors== (A) Representative immunocytochemistry of pluripotency markers POU5F1 (OCT4), NANOG, TRA-1-81, and SSEA4 in hiPSC line CBiPSC6.2 after >20 passages. (B) |
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