File:Chromosome motility anaphase.jpg

Chromosome motility anaphase

Model: Katanin, Spastin, and Fidgetin function distinctly to drive Pacman-flux chromosome motility during anaphase.

(A) At centrosomes, Spastin and Fidgetin sever MTs from the protective ?-TuRCs associated with centrosomes/poles. The newly generated MT ends can now disassemble; minus-end disassembly of the released MT is promoted by Kinesin-13 at the poles. Plus-end disassembly eliminates the short remaining MT and allows ?-TuRCs turnover at centrosomes.

(B) At chromosomes, Katanin severs near the plus ends of spindle MTs, generating new free ends. This activity would remove any protective caps stabilizing plus ends. Kinesin-13 at centromeres/kinetochores then stimulates minus-end disassembly, enabling chromosomes to move poleward by Pacman.

Reference

<pubmed>17452528</pubmed>

Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes. Zhang D, Rogers GC, Buster DW, Sharp DJ. J Cell Biol. 2007 Apr 23;177(2):231-42. PMID:17452528

Beginning six months after publication, RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.

© The Rockefeller University Press $15.00 The Journal of Cell Biology, Vol. 177, No. 2, April 23, 2007 231–242 http://www.jcb.org/cgi/doi/10.1083/jcb.200612011

Figure 9.