File:Chicken neural Plxdc2 expression.jpg

Analysis of Plxdc2 in the developing chick brain

In the chick, Plxdc2 expression was found to be similar to that previously documented in the mouse [9]. a, b, e and f, still images from different external viewing angles of volume rendered specimens showing the orientation of virtual sections in c, d and g–i. c and d, Plxdc2 expression was absent from the region of the cortical hem at HH stage 20 (arrow: b, c and d). g–i, Plxdc2 expression in the cortical hem and surrounding mesoderm was evident by HH stage 21 (arrow: f, g and i). j–l, Plxdc2 in situ hybridisation of coronal sections through the brain of a HH stage 36 chick embryo. m, higher magnification image of indicated region of l showing Plxdc2 expression in the Purkinje cell layer and a lack of Plxdc2 expression in granule cell raphes (arrowheads). Arrowhead in c, floor of midbrain; b, branchial arch expression; ot, otic vesicle; MDB, surrounding mesenchyme of the mesencephalon-diencephalon boundary; MHB, midbrain-hindbrain boundary; Arrow in k and l, Plxdc2 expression in the neuroepithelium; cb, cerebellum; fp, floor plate; med, medulla oblongata; N5n, trigeminal motor nucleus; PCL, Purkinje Cell Layer; Pr5, principle sensory trigeminal nucleus; VI, abducens nucleus.

Scale bar: a–d, 0.4 mm; e–i, 0.5 mm; j–l, 1 mm; m, 250 µm.

Original file name: Figure 1. Journal.pone.0014565.g001.png (original image scaled to 1000px and converted to jpg)

Reference

<pubmed>21283688</pubmed>| PMC3024984 | PLoS One

Citation: Miller-Delaney SFC, Lieberam I, Murphy P, Mitchell KJ (2011) Plxdc2 Is a Mitogen for Neural Progenitors. PLoS ONE 6(1): e14565. doi:10.1371/journal.pone.0014565

Copyright: © 2011 Miller-Delaney et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.