Category:Carnegie Embryo 8963

From Embryology
Carnegie Embryo 8963

This Embryology category shows pages and images that relate to the Carnegie Collection Embryo No.8963. This 22 somite embryo was a tubal pregnancy originally classified as University of Chicago No. H1093. Described by Wen (1928)[1] with particular reference to the neural, renal, vascular systems.

Carnegie stage 12 - Week 4, 26 - 30 days, GA - week 6, 3.8 mm. (dors. flex.), Somite Number 22. 14.5 x 11 x 7 mm.


Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
8963 22 E, 3.8 Ch , 14.5 Fair Formol C-P Trans 10 I.H. 1928 Univ. Chicago. No. H 1093 Studied by Wen (1928)[1]
Carnegie Collection Embryos - Stage 12 
Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
209 ca_24 EH3 Ch.,15 Poor Alc P Coronal 50 Al. coch. 1902
250 19? E , 2 (11, 10x9x9 Poor p ? Sagittal 20 Al. coch. p
384 P E 2_5 Ch.,13 Poor Formol P Transverse 10 H.&E. 1907 Macerated. Narrow yolk stalk
486 21 E.,4 Ch., 22 Good Corros. acetic P Transverse 10 Al. Coch. 1911
1062 29 E.,4.5 Ch., 20 Good Formol P Transverse 20 Al. coch. 1915 Transitional to next stage
2197 ? E_,5_3 Ch., 19.5 Poor Formol P Transverse 10 Al. coch, or. G. 1918
4245-7 ca.24 E.,3.5 Ch., 24 Good Alc, formol P Transverse 10 Al. coch. 1923 Caudal neuropore widely open
4479 P E.,5 .8 Ch , 17 Poor Formol P Transverse 40 Al. coch. 1923 Macerated. Upper lirnb buds not visible
4736 26 E.,3.0 Ch.,20 Good Formol P Coronal 10 Al. coch. 1924 No upper limb buds. Caudal neuropore closed
4759 ? E.,4.5 Ch.,15 Good Formol P Transverse 15 (Stain - Haematoxylin Eosin) 1924 Neural tube folded
4784 23 E_,3 Good P P Transverse 10 p 1924
5035 25-28 E.,3.8 Ch.,18 Good Formol C-P Transverse 10 Al. coch. 1925
5048 ca_25 E_,3_5 Good Formol C-P Transverse 10 Al. coch. 1925 Tubal Injured
5056 25 E,,3 Ch.,12 Good Formol P Transverse 10 Al. coch. 1925
5206 ? E.,4 Ch., 51x31x30 Poor ?? P Transverse 20 Al. coch. 1926 Tubal
5300 ? E., 4.5 Ch,16.5 Poor Formol P Transverse 20 Al. coch. 1926 Autopsy. Partly macerated
5923 28 E.,4 Ch.,15 Exc. Formol P Transverse 10 Al. coch. 1929
6097 25 E,3.4 Ch., 12.5 Exc. Formol C-P Transverse 10 Al. coch, eosin 1930 Tubal Ag added to slides 1-3
6144 27 E. 3.3 Ch.,11 Good Lysol—Zenker C—P Transverse 10 Al. coch. 1930
6488 28 E, 32 Ch,22 Good Formol C—P Transverse 10 Al. coch. 1932
6937 26 E.,3 Ch , 12 Poor Formol C—P Coronal 10 I.H.,or.G. 1934 Tubal Caudal neuropore closed
7724 ca.29 E,3.5 Ch.,18 Good Formol C—P Sagittal 8 (Stain - Haematoxylin Eosin) 1940 Caudal end broken
7852 25 E , 3.7 Ch,26 Exc. Formol C—P Transverse 10 (Stain - Haematoxylin Eosin) 1940 Typical for stage 12
7999 ca.28 E,3.2 Ch , 15 Exc. Bruin C-P Transverse 10 (Stain - Haematoxylin Eosin) 1942 Caudal defect
8505a 24 Ch, 23.5 Exc. Formol C-P Transverse P H. Phlox. 1947
8505b 23 Ch,24 Exc. Formol C-P Sagittal p Azan 1947 Twins
8941 28 E,4.9 Ch, 35 Exc. Zenker C-P Transverse 6 I.H. 1927 Univ. Chicago No. H 1261
8942 25 E, 38 Ch, 35 Exc. Formal-Zenker C-P Coronal 5 11-1. 1930 Univ. Chicago No. H 1382
8943 22 E. 3.9 Ch, 20.4 Exc. Formal-Zenker C-P Transverse 8 H.&E. 1934 Univ. Chicago No. H 1481
8944 25 E,4 Ch,,25 Exc. Formal-Zenker C-P Sagittal 8 I.H. 1936 Univ. Chicago No. H 1514
8963 22 E, 3.8 Ch , 14.5 Fair Formol C-P Transverse 10 I.H. 1928 Univ. Chicago. No. H 1093 Studied by Wen (1928)[1]
8964 23 E,2.8 Ch - 25 Poor Formol p Transverse 8 I.H. 1928 Univ_ Chicago No. H984 Studied by Wen (1928)[1]
9154 24 E, 5 4 Exc Formal C-P Transverse I.H. & phlox. 1953
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  •  ? - unknown or not determined.
References


The embryo was sectioned into 323 sections at 10 µ.


Embryo H1093 (fig. 1, B) was obtained by Dr. Garrette Van Sweringen and Dr. W. W. Duemling, of Fort Wayne, Indiana. The clinical record gives the following points: “The October period began on the 6th, 1924; next period from 10th to 14th or 15th. The expected period on December 10th did not appear. Flow supposed to be menstruation appeared on December 22nd, but bloody discharge continued until 31st. Contraceptive measures were employed until after November period. Symptoms of ectopic pregnancy were observed on January 4, 1925, and operation was performed on the same day.” In cutting open the hematoma, the head of the embryo was severed from the trunk just caudal to the otocyst. Although no loss of tissue is apparently involved, yet the difference in the plane of sections of the two portions of the embryo makes it materially difficult to follow the series in the region of rhombomere VI. The specimen was fixed in neutral formalin, double embedded, cut transversely, and stained with iron hematoxylin. Histological differentiation is fair, but there is much shrinkage. Cells with coarse granules evidently representing necrobiosis are found abundant in the neural crest as well as the medullary tube. A wax model of the nervous system in front of the otocyst was made from serial photomicrographs at the magnification of 150 times.[1]


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