Category:Carnegie Embryo 4315

From Embryology
Carnegie Embryo 4315

This Embryology category shows pages and images that relate to the Carnegie Collection Embryo No.8963. This 22 somite embryo was a tubal pregnancy originally classified as University of Chicago No. H951. Described by Wen (1928)[1] with particular reference to the neural, renal, vascular systems.

"Embryo H951 (fig. 1, A) was obtained from Drs. E. L. Whitney and J. T. Rocks, of Walla Walla, Washington. “The uterus was removed for fibrosis on the 13th day after the day on which menstruation was expected to begin,” opened and fixed in ‘4%’ formalin. No further clinical information could be obtained. The embryo was mordanted in aqueous chrom—sublimate, slowly dehydrated, double embedded in celloidin and paraffin, cut into transverse sections, and stained with iron hematoxylin (all but one slide being counterstained with eosin). During the mounting, five sections were slightly distorted and, unfortunately, three sections lost, so it is not easy to follow the series at the region between the 4th to 5th somites. A plaster model of the head region (figs. 2, 3), a wax model of the entire embryo, and one of the anterior portion of the central nervous system with the ventral pharyngeal wall were made from serial photomicrographs at the magnification of 150 times. Two other plaster models, one of the entire embryo and one of the head region alone showing the anatomy therewith, were made by O. O. Heard. Histologically, this specimen is in excellent condition."[1]

Carnegie stage 11 - Week 4, 23 - 26 days, GA - week 6, 4.0 mm GL, Somite Number 17. Conceptus - 23.1 x 10.4 x circ.11 mm.


The embryo was sectioned into 336 sections at 10 µm.

Carnegie Collection - Stage 11 
Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
4315 17 E, 4.7 Ch, 23x10.4X11 Excellent  ? C-P Transverse 10 I. (Stain - Haematoxylin Eosin) 1923 Univ. Chicago No. 951. Wen (1928)[1]
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  •  ? - unknown or not determined.
Carnegie Collection - Stage 11 
Serial No. Pairs of somites Size (mm) Grade Fixative Embedding Medium Plane Thinness (µm) Stain Year Notes
12 14 E, 2.1 Ch, 13 Poor P Transverse 10 Al. carm. 1893
164 18 E, 3.5 Ch, 14 Good Formol P Transverse 20 Al. carm. 1913
318 13/14 E, 2.5 Ch, 16 Good P Transverse 25 Al. carm. 1905
470 17 E, 4.3 Ch, 16 Good Formol P Transverse 10 Al. carm. . 1910
779 14 E, 2.75 Good C Transverse 15 Al. coch. 1913 Dysraphism. Noted by Dekaban (1964)[1]
1182b E, 3 Ch, 15x12x5 Good Formol  ? Transverse 20 Al. carm. 1915
2053 20 E, 3.1 Ch, 12 Exc. Formol P Transverse 10 Al. coch. 1918 Most advanced in group. Ag added to slide 2
Monographs by Davis (1923)[2] and Congdon (1922)[3]
4315 17 E, 4.7 Ch, 23x10.4X11 Excellent  ? C-P Transverse 10 I.H. & E. 1923 Univ. Chicago No. 951. Wen (1928)[4]
4529 14 E, 2.4 Ch, 21 Excellent Formol P Transverse 10 Al. coch, or. G. 1924 Heuser (1930)[5]
4783 13 E, 2.3 Fair  ?  ? Transverse 5 I.H. 1924 Wallin (1913)[6]
4877 13 E, 2 Ch, 15 Good Formol P Transverse 15 Al. coch. 1925
5072 17 E, 3 Good Formol P Transverse 10 (Stain - Haematoxylin Eosin) 1925 Tubal Type specimen. Atwell (1930)[7]
6050 19/21 E.,3 Ch, 10 Good Formol C-P Coronal 10 Al. coch. 1930 Advanced
6344 13 E, 2.5 Ch, 17 Excellent Formol C-P Transverse 6 Al. coch. 1931 Least advanced in group
6784 17 E, 5 Ch, 16 Excellent Formol C-P Transverse 6 I.H, or. G. 1933
7358 16 E, ? Ch, 15 Poor Alc, formol p Oblique 25 (Stain - Haematoxylin Eosin) 1936
7611 16 E., 2.4 Ch., 12 Excellent Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1938
7665 19 E., 4.36 Excellent  ? C-P Transverse 6 1939 Univ. Chicago No. H 1516
7702 17 E, 3.7 Ch., 14 Good Formol C-P Transverse 10 Al. coch. 1940 Returned to B M Patten
7851 13 E., 4.3 Ch, 18 Excellent Formol C-P Transverse 8 (Stain - Haematoxylin Eosin) 1940 Slightly injured
8005 16/17 E, 3 Excellent Bouin C-P Transverse 8 (Stain - Haematoxylin Eosin) 1942 Tubal
8116 17 E, 14 Ch.. 17 Good Formol p Sagittal 8 Azan 1953
8962 15 E, 1.55 Good  ? * Sagittal  ?  ? 1952 Tubal Univ. Chicago No. H 810
Abbreviations
  • Size - E. is the greatest length of the embryo and Ch. is the mean diameter of the chorion.
  • Grade - total grade of the specimen and includes both its original quality and the condition of the mounted sections.
  • Embedding medium - paraffin (P) or a combination of celloidin and paraffin (C-P).
  • Fixative - formalin (Formol), alcohol and formalin (Alc, formol), Bouin (Bouin solution)
  •  ? - unknown or not determined.
References
  1. Dekaban AS. and Bartelmez GW. Complete Dysraphism in 14 Somite Human Embryo. (1964) A Contribution To Normal And Abnormal Morphogenesis. Am. J. Anat. 115: 27-38. PMID 14199785
  2. Davis CL. Description of a human embryo having twenty paired somites. (1923) Carnegie Instn. Wash. Publ. 332, Contrib. Embryol., 15: 1-51.
  3. Congdon ED. Transformation of the aortic-arch system during the development of the human embryo. (1922) Contrib. Embryol., Carnegie Inst. Wash. Publ 277, 14:47-110.
  4. Wen IC. The anatomy of human embryos with seventeen to twenty-three pairs of somites (1928) J. Comp. Neural., 45: 301-376.
  5. Heuser CH. A human embryo with 14 pairs of somites. (1930) Carnegie Instn. Wash. Publ. 414, Contrib. Embryol., Carnegie Inst. Wash. 22:135-153.
  6. Wallin IE. A human embryo of thirteen somites. (1913) Amer. J Anat. 15(3): 319-331.
  7. Atwell WJ. A human embryo with seventeen pairs of somites. (1930) Contrib. Embryol., Carnegie Inst. Wash. Publ. 407, 21: 1-24.

Pages in category ‘Carnegie Embryo 4315’

This category contains only the following page.

Media in category ‘Carnegie Embryo 4315’

The following 19 files are in this category, out of 19 total.