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Staining solutions | |||
'''Staining solutions''' | |||
A. Hematoxylin (CI No. 75290), 0.5 gm; ferric ammonium sulfate, 1.0 gm; 0.6 N H$Ol (aq.), 50 ml; 95% ethanol, 50 ml. Filter when solution is complete. The pH should be approximately 1.6. The solution is usable 10 min after preparation but gives best results in 4-8 wk. | A. Hematoxylin (CI No. 75290), 0.5 gm; ferric ammonium sulfate, 1.0 gm; 0.6 N H$Ol (aq.), 50 ml; 95% ethanol, 50 ml. Filter when solution is complete. The pH should be approximately 1.6. The solution is usable 10 min after preparation but gives best results in 4-8 wk. | ||
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C. Fast green FCF (CI No. 42053), 0.5 gm; 95% ethanol, 100 ml. p H 3.6. | C. Fast green FCF (CI No. 42053), 0.5 gm; 95% ethanol, 100 ml. p H 3.6. | ||
Staining procedure | '''Staining procedure''' | ||
# Remove embedding material and hydrate to 70% ethanol. | # Remove embedding material and hydrate to 70% ethanol. | ||
# Stain in solution A for 15-30 min. | # Stain in solution A for 15-30 min. | ||
# Wash in distilled water. | # Wash in distilled water. | ||
# Destain brie5y in acid alcohol consisting of: 0.6 N H,S04, 10 ml; distilled water, 50 ml; and 95% ethanol, 40 ml. | # Destain brie5y in acid alcohol consisting of: 0.6 N H,S04, 10 ml; distilled water, 50 ml; and 95% ethanol, 40 ml. | ||
# Place for 2 min in a solution consisting of: NaHCO,, 0.5 gm; distilled | # Place for 2 min in a solution consisting of: NaHCO,, 0.5 gm; distilled water, 50 ml; and 95% ethanol, 50 ml. | ||
water, 50 ml; and 95% ethanol, 50 ml. | # Stain in solution B for 15-30 min. The staining time may be adjusted to suit the material. | ||
# Stain in solution B for 15-30 min. The staining time may be adjusted to | |||
suit the material. | |||
# Wash in 95% ethanol to remove excess stain. | # Wash in 95% ethanol to remove excess stain. | ||
# Stain in solution C for 15 sec. | # Stain in solution C for 15 sec. | ||
Revision as of 11:54, 15 August 2011
Staining solutions
A. Hematoxylin (CI No. 75290), 0.5 gm; ferric ammonium sulfate, 1.0 gm; 0.6 N H$Ol (aq.), 50 ml; 95% ethanol, 50 ml. Filter when solution is complete. The pH should be approximately 1.6. The solution is usable 10 min after preparation but gives best results in 4-8 wk.
B. Biebrich scarlet WS (CI No. 26905), 0.25 gm; orange G (CI No. 16230), 0.25 gm; phosphotungstic acid, 0.5 gm; phosphomolybdic acid, 0.5 gm; distilled water, 50 ml; 95% ethanol, 50 ml. p H 2.6.
C. Fast green FCF (CI No. 42053), 0.5 gm; 95% ethanol, 100 ml. p H 3.6.
Staining procedure
- Remove embedding material and hydrate to 70% ethanol.
- Stain in solution A for 15-30 min.
- Wash in distilled water.
- Destain brie5y in acid alcohol consisting of: 0.6 N H,S04, 10 ml; distilled water, 50 ml; and 95% ethanol, 40 ml.
- Place for 2 min in a solution consisting of: NaHCO,, 0.5 gm; distilled water, 50 ml; and 95% ethanol, 50 ml.
- Stain in solution B for 15-30 min. The staining time may be adjusted to suit the material.
- Wash in 95% ethanol to remove excess stain.
- Stain in solution C for 15 sec.
