File:Patterns of ZPC Deposition in Porcine Oocytes.jpg: Difference between revisions
(==Patterns of ZPC Deposition in Porcine Oocyte-Cumulus Complexes== The zona pellucida protein, ZPC, is a receptor for the spermatozoon in fertilisation<ref name="PMID21383844"><pubmed>21383844</pubmed></ref>. Immunofluorescence detection for ZPC and u) |
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==Patterns of ZPC Deposition in Porcine Oocyte-Cumulus Complexes== | ==Patterns of ZPC Deposition in Porcine Oocyte-Cumulus Complexes== | ||
The zona pellucida protein, ZPC, is a receptor for the spermatozoon in fertilisation<ref name="PMID21383844"><pubmed>21383844</pubmed></ref>. | The zona pellucida protein, ZPC, is a receptor for the spermatozoon in fertilisation<ref name="PMID21383844"><pubmed>21383844</pubmed></ref>. | ||
Immunofluorescence detection for ZPC and ubiquitin in a porcine oocyte<ref name="PMID21383844"/>. | |||
(a, b) ZPC (grey) detected in ridges (arrowheads) adjacent to zona-adhering corona radiata cells (nuclei stained blue). | (a, b) ZPC (grey) detected in ridges (arrowheads) adjacent to zona-adhering corona radiata cells (nuclei stained blue). | ||
(c-e) ZPC (red) and ubiquitin (green) detected in the ZP. | (c-e) ZPC (red) and ubiquitin (green) detected in the ZP. | ||
(c, d) ZPC (red) and ubiquitin (green) in the cytoplasm of corona radiata cells. | (c, d) ZPC (red) and ubiquitin (green) in the cytoplasm of corona radiata cells. | ||
(e) DNA for ZPC counterstained with DAPI (blue). | (e) DNA for ZPC counterstained with DAPI (blue). | ||
(f) Western blotting of ZPC protein in the isolated cumulus/corona cells from 60 oocyte cumulus complexes (lane 1), in 60 zona-free oocytes (lane 2) and in soluble zona proteins isolated from 60 oocytes (lane 3). | (f) Western blotting of ZPC protein in the isolated cumulus/corona cells from 60 oocyte cumulus complexes (lane 1), in 60 zona-free oocytes (lane 2) and in soluble zona proteins isolated from 60 oocytes (lane 3). | ||
Revision as of 12:30, 4 August 2012
Patterns of ZPC Deposition in Porcine Oocyte-Cumulus Complexes
The zona pellucida protein, ZPC, is a receptor for the spermatozoon in fertilisation[1].
Immunofluorescence detection for ZPC and ubiquitin in a porcine oocyte[1].
(a, b) ZPC (grey) detected in ridges (arrowheads) adjacent to zona-adhering corona radiata cells (nuclei stained blue).
(c-e) ZPC (red) and ubiquitin (green) detected in the ZP.
(c, d) ZPC (red) and ubiquitin (green) in the cytoplasm of corona radiata cells.
(e) DNA for ZPC counterstained with DAPI (blue).
(f) Western blotting of ZPC protein in the isolated cumulus/corona cells from 60 oocyte cumulus complexes (lane 1), in 60 zona-free oocytes (lane 2) and in soluble zona proteins isolated from 60 oocytes (lane 3).
References
Copyright: © 2011 Zimmerman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Original file name: Figure 6. doi:10.1371/journal.pone.0017256.g006 http://www.plosone.org/article/info:doi/10.1371/journal.pone.0017256#pone-0017256-g006
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