ANAT2341 Lab 4

2. Guest Lecturer - Dr Fabien Delerue

Dr Fabien Delerue

Manipulating the mouse embryo: from ES Cells to genome editing Dr Delerue is currently in the Transgenic Animal Unit (TAU) his career spans over twenty years practice in animal handling, surgery, and generation of animal models of diseases. His research focuses on the production and characterization of new transgenic mouse models of human genetic disorders, using cutting-edge genome engineering techniques such as CRISPR/Cas9. Links: UNSW Research Gateway

Lecture slides: File:Delerue.pdf

References

Delerue F & Ittner LM. Genome Editing in Mice Using CRISPR/Cas9: Achievements and Prospects Cloning and Transgenesis 2015, 4:135 doi:10.4172/2168-9849.1000135 [1]

"Animal models are a powerful tool to understand the mechanisms underlying physiological and pathological processes in vivo. To date, mice remain the species most commonly used for genetic manipulation. The recent development of engineered endonucleases such as Zinc Finger Nucleases (ZFN), Transcription activator-like effector nucleases (TALEN), and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) offered easy, flexible, and fast alternative to ES-Cell based gene targeting. Thanks to multiple advantages, the CRISPR system superseded its predecessors and became a popular method for genome editing. Here, we review the latest techniques to apply CRISPR editing to the mouse genome, and emphasize on the current methods used in transgenic laboratories and subsequent achievements in mice."

Ke YD, van Hummel A, Stevens CH, Gladbach A, Ippati S, Bi M, Lee WS, Krüger S, van der Hoven J, Volkerling A, Bongers A, Halliday G, Haass NK, Kiernan M, Delerue F, Ittner LM.
Short-term suppression of A315T mutant human TDP-43 expression improves functional deficits in a novel inducible transgenic mouse model of FTLD-TDP and ALS. Acta Neuropathologica 2015 Nov;130(5):661-78 PubMed 26437864 [2]

Ittner A, Chua SW, Bertz J, Volkerling A, van der Hoven J, Gladbach A, Przybyla M, Bi M, van Hummel A, Stevens CH, Ippati S, Suh LS, Macmillan A, Sutherland G, Kril JJ, Silva APG, Mackay J, Poljak A, Delerue F, Ke YD, Ittner LM. Site-specific phosphorylation of tau inhibits amyloid-β toxicity in Alzheimer’s mice Science 2016 Nov;354 (6314):904-908 doi: 10.1126/science.aah6205 [3]

Recommended Literature

Manipulating the Mouse Embryo: A Laboratory Manual (Fourth Edition) By Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, & Andras Nagy.	Reproductive Engineering techniques in mice: Technical manual Prof Naomi Nakagata, Center for Animal Resources and Development, Kumamoto University, Japan

External Links

External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.

• NCBI Search - NCBI databases - CRISPR | PubMed CRISPR | PubMed Centrap CRISPR
• JoVE - [4]

<html5media height="460" width="640">https://www.youtube.com/watch?v=16g6fEup2pQ</html5media>

3. Student Group Projects

I have now added (I hope) a discussion Forum for your group to Moodle available to only your group members.

2017 Project Groups
Group 1	Group 2	Group 3	Group 4	Group 5	Group 6
Z5177691 Z5178570 Z5093005 Z5059696 Z5059949	Z5178275 Z5178407 Z5076039 Z5017644 Z5015446	Z5178463 Z5076019 Z5059996 Z5076466 Z5018962	Z5177670 Z5117343 Z5075309 Z5075778 Z3416557	Z5178462 Z5059373 Z5114217 Z5062492 Z5076351	Z5177699 Z5113034 Z5114433 Z5076158 Z5018156
				Mark Hill - Lab 1 page