Mouse Thymus Movie 1: Difference between revisions

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Adult Mouse Thymus - T cell Elimination

<html5media height="530" width="490">File:Mouse adult thymus 01.mp4</html5media>

Click Here to play on mobile device

Dying thymocytes are efficiently cleared by phagocytes.

Examples of cell death in situ during negative selection.

Purified CD4+CD8+ F5 thymocytes labeled with SNARF and Hoechst were introduced into LysM-GFP thymic slices then treated with 1 nM specific peptide for 30 min.

The incubation was continued for the indicated times and the slices were imaged by time-lapse two-photon microscopy.

The arrowheads point to dying thymocytes. Scale bars are 5 µm.

Links: Thymus Development | Immune | Movies

Reference

Dzhagalov IL, Chen KG, Herzmark P & Robey EA. (2013). Elimination of self-reactive T cells in the thymus: a timeline for negative selection. PLoS Biol. , 11, e1001566. PMID: 23700386 DOI.

Copyright

© 2013 Dzhagalov et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

doi:10.1371/journal.pbio.1001566.s006 (MOV)

http://journals.plos.org/plosbiology/article/asset?unique&id=info:doi/10.1371/journal.pbio.1001566.s006

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Cite this page: Hill, M.A. (2024, June 1) Embryology Mouse Thymus Movie 1. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Thymus_Movie_1

What Links Here?

@@ Line 1: / Line 1: @@
 {{Movie header}}
-==Adult Mouse Lymph Node - T cell motility and transendothelial migration==
+==Adult Mouse Thymus - T cell Elimination==
 {|
 |-
-| width=420px|<html5media height="380" width="400">File:Mouse_adult_lymph_node_01.mp4</html5media>
+| width=500px|<html5media height="530" width="490">File:Mouse adult thymus 01.mp4</html5media>
+[[Media:Mouse adult thymus 01.mp4|'''Click Here''' to play on mobile device]]
+| Dying thymocytes are efficiently cleared by phagocytes.
-[[Media:Mouse_adult_lymph_node_01.mp4|'''Click Here''' to play on mobile device]]
+Examples of cell death in situ during negative selection.
-| [[File:Lymph_node_cartoon_02.jpg|alt=Lymph node cartoon|500px]]
-|-
-| [[File:Mouse adult lymph node 01.jpg]]
-[[Media:Mouse_adult_lymph_node_01.mp4|Open Movie in new window]]
+Purified CD4+CD8+ F5 thymocytes labeled with SNARF and Hoechst were introduced into LysM-GFP thymic slices then treated with 1 nM specific peptide for 30 min.
-| This movie shows T cell (green) motility and transendothelial (outlined by white dots) migration.
-|-
-|}
+The incubation was continued for the indicated times and the slices were imaged by time-lapse two-photon microscopy.
-{{Movie footer}}
+The arrowheads point to dying thymocytes. Scale bars are 5 µm.
+:'''Links:''' [[Thymus Development]] | [[Immune System Development|Immune]] | [[Movies]]
-===Elimination of self-reactive T cells in the thymus: a timeline for negative selection===
+[[File:Mouse adult thymus 11.jpg|300px]]
-PLoS Biol. 2013;11(5):e1001566. doi: 10.1371/journal.pbio.1001566. Epub 2013 May 21.
-Dzhagalov IL1, Chen KG, Herzmark P, Robey EA.
+|}
+[[File:Mouse adult thymus 01.jpg|thumb]]
+===Reference===
-Abstract
+{{#pmid:23700386}}
-The elimination of autoreactive T cells occurs via thymocyte apoptosis and removal by thymic phagocytes, but the sequence of events in vivo, and the relationship between thymocyte death and phagocytic clearance, are unknown. Here we address these questions by following a synchronized cohort of thymocytes undergoing negative selection within a three-dimensional thymic tissue environment, from the initial encounter with a negative selecting ligand to thymocyte death and clearance. Encounter with cognate peptide-MHC complexes results in rapid calcium flux and migratory arrest in auto-reactive thymocytes over a broad range of peptide concentrations, followed by a lag period in which gene expression changes occurred, but there was little sign of thymocyte death. Caspase 3 activation and thymocyte loss were first detectable at 2 and 3 hours, respectively, and entry of individual thymocytes into the death program occurred asynchronously over the next 10 hours. Two-photon time-lapse imaging revealed that thymocyte death and phagocytosis occurred simultaneously, often with thymocytes engulfed prior to changes in chromatin and membrane permeability. Our data provide a timeline for negative selection and reveal close coupling between cell death and clearance in the thymus.
+====Copyright====
-Comment in
+© 2013 Dzhagalov et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
-Bringing you negative selection, live and in color. [PLoS Biol. 2013]
-PMID 23700386
+doi:10.1371/journal.pbio.1001566.s006 (MOV)
-http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001566
+http://journals.plos.org/plosbiology/article/asset?unique&id=info:doi/10.1371/journal.pbio.1001566.s006
-http://journals.plos.org/ploscollections/article?id=10.1371/journal.pbio.1001566
+{{Movie footer}}
+[[Category:Immune]][[Category:Mouse]][[Category:Thymus]]