Royal Hospital for Women - Reproductive Medicine Seminar 2018: Difference between revisions
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* '''Week 6''' - fetal cortex, from mesothelium adjacent to dorsal mesentery; Medulla, neural crest cells from adjacent sympathetic ganglia | * '''Week 6''' - fetal cortex, from mesothelium adjacent to dorsal mesentery; Medulla, neural crest cells from adjacent sympathetic ganglia |
Revision as of 11:38, 27 February 2018
Embryology - 3 Jun 2024 Expand to Translate |
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Google Translate - select your language from the list shown below (this will open a new external page) |
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Introduction
This is the partial online draft version of my presentation, as there was no internet I switched to powerpoint fversion or the final presentation. I will though update this online version as it will include full access to molecular data and all animations presented in my talk.
Powerpoint Slides: 1 slide/page PDF | 4 slides/page PDF
Requested Lecture Content |
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Dr Rachael Rodgers - "Male and female reproductive/urogenital systems, breast, thyroid, adrenals, kidneys, hypothalamus and pituitary, it would be perfect." |
Background
- Uterine Tube Biobank (Prof Ledger)
- Molecular aspects of ectopic implantation
- Trophoblast differentiation
- Control of implantation and early placentation
- Digital Embryology Consortium
- The objective of this international partnership is to digitise, preserve, and make available for researchers the major embryology histological collections.
- Kyoto Collection(eBook)
- with Shiota, Yamada and Ho.
- Human SEM (eBook)
- in preparation with Sulik, Vekemans and Attié-Bitach.
- UNSW Embryology
Reproductive
Timeline
Gestational Age GA Week |
Event | Fertilization Age Week |
---|---|---|
5-6 | primordial germ cells migrate during gastrulation | 3-4 |
6 | intermediate mesoderm, pronephros primordium | 4 |
7 | mesonephros and mesonephric duct (Wolffian duct) | 5 |
8 | ureteric bud, metanephros, genital ridge | 6 (35 days) |
9 | cloacal divison, gonadal primordium - indifferent to first appearance of testis cords | 7 (42 days) |
10 | paramesonephric duct (Mullerian duct), clear gonadal differentiation | 8 (49 days) |
11 | paramesonephric duct fusion (female) | 9 (56 days) |
17 | primary follicles (ovary) | 15 (100 days) |
Primordial Germ Cells
Primoridal Germ Cell Migration
Primordial Germ Cells (PGCs) are thought to be the first population of cells to migrate through the primitive streak in early gastrulation.
Human Embryonic Disc (Stage 7 GA week 5)
GA Week 5
- Human embryonic disc showing the primitive streak region where gastrulation occurs, generation the trilaminar embryo.
- Arrows indicate direction of cell migration through the streak.
This population of cells then lie at the hindgut and yolk sac junctional region and later migrate into the germinal ridge in early embryonic development.
Human Embryo (Stage 9 GA week 5) primordial germ cell region (green)
- Sacrococcygeal teratomas - Remnant primitive streak cells (most common solid tumor in newborn infants)
- Germline teratoma - (Germinoma) abnormally differentiated/located PGCs fail to die.
Mouse PGC Migration
E9.5 | E10.5 |
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<html5media height="400" width="400">File:Primordial_germ_cell_002.mp4</html5media> |
<html5media height="400" width="400">File:Primordial_germ_cell_003.mp4</html5media> |
PGC motility 3 phases - initiation, migration and stopping
- based upon normal actin cell motility.
- no sex-specific differences
PGC Chemoattraction
Chemoattraction
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Stromal Derived Factor 1 (SDF-1, CXCL12) chemotaxis
<html5media width="400" height="300">https://www.youtube.com/embed/tqlc_YAtWRE</html5media> |
Intermediate Mesoderm
Mesoderm Generation (chicken)[3]
<html5media height="700" width="300">File:Chicken presomitic mesoderm 03.mp4</html5media> |
Human trilaminar embryo {"Germ layers")
Intermediate mesoderm derives genital and renal systems. |
<html5media height="520" width="360">File:Urogenital_sinus_001.mp4</html5media> GA week 6-7 Urogenital Sinus Movie |
It is not the primordial germ cells which respond to SRY presence or absence, but the supporting cells within the developing gonad.
Urogenital Septum
<html5media height="440" width="420">File:Urogenital_septum_001.mp4</html5media> |
GA Week 8
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Mouse Gonad
Sertoli and Germ Cells - showing PGCs are not responsive to SRY signaling.
Sry Signaling[4]
- red - Sertoli cells, showing Fgf9 expression (following Sry expression FGF9 is a downstream signaling molecule).
- green - Germ cells and endothelial cells, showing PECAM expression.
Genital Ridge (early embryo)
- green - gonadal supporting cells
- blue - Sertoli cells (male)
- pink - pre-granulosa cells (female)
RSPO1 R-Spondin Family, Member 1
Sex Development Genes
Gene (OMIM) | Protein Function | Gonad Phenotype of Null Mice | Human Syndrome | |
Bipotential gonad | ||||
Wt1 | Transcription factor | Blockage in genital ridge development | Denys-Drash, WAGR, Frasier syndrome | |
Sf1 | Nuclear receptor | Blockage in genital ridge development | Embryonic testicular regression syndrome | |
Lhx9 | Transcription factor | Blockage in genital ridge development | a | |
Emx2 | Transcription factor | Blockage in genital ridge development | a | |
M33 | Transcription factor | Gonadal dysgenesis | a | |
Testis-determining pathway | ||||
Gata4/Fog2 | Transcription/cofactor | Reduced Sry levels, XY sex reversal | a | |
Sry | Transcription factor | XY sex reversal | XY sex reversal (LOF); XX sex reversal (GOF) | |
Sox9 | Transcription factor | XY sex reversal | Campomelic dysplasia, XX sex reversal (GOF) | |
Sox8 | Transcription factor | XY sex reversal in combination with partial loss of Sox9 function | a | |
Fgf9 | Signaling molecule | XY sex reversal | a | |
Dax1 | Nuclear receptor | Impaired testis cord formation and spermatogenesis | Hypogonadism | |
Pod1 | Transcription factor | XY sex reversal | a | |
Dhh | Signaling molecule | Impaired differentiation of Leydig and PM cells | XY gonadal dysgenesis | |
Pgdra | Receptor | Reduction in mesonephric cell migration | a | |
Pgds | Enzyme | No phenotype | a | |
Arx | Transcription factor | Abnormal testicular differentiation | X-linked lissencephaly with abnormal genitalia | |
Atrx | Helicase | ND | ATRX syndrome | |
Insl3 | Signaling factor | Blockage of testicular descent | Cryptorchidism | |
Lgr8 | Receptor | Blockage of testicular descent | Cryptorchidism | |
Hoxa10 | Transcription factor | Blockage of testicular descent | Cryptorchidism | |
Hoxa11 | Transcription factor | Blockage of testicular descent | Cryptorchidism | |
Amh | Hormone | No Müllerian duct degeneration | Persistent Müllerian duct syndrome | |
Misrl1 | Receptor | No Müllerian duct degeneration | Persistent Müllerian duct syndrome | |
Pax2 | Transcription factor | Dysgenesis of mesonephric tubules | a | |
Lim1 | Transcription factor | Agenesis of Wolffian and Müllerian ducts | a | |
Dmrt1 | Transcription factor | Loss of Sertoli and germ cells | XY femaleb | |
Ovary-determining pathway | ||||
Wnt4 | Signaling molecule | Müllerian duct agenesis, testosterone synthesis, and coelomic vessel formation | XY female (GOF) | |
FoxL2 | Transcription factor | Premature ovarian failure | BPES | |
Dax1 | Nuclear receptor | XY sex reversal (GOF) | XY sex reversal (GOF) | |
RSPO1 | Signaling molecule | XX sex reversal (LOF) | XX sex reversal (LOF) | |
Table Legend | ||||
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a No mutations in human sexual disorders identified to date.
b Candidate gene for 9p deletion, XY sex reversal. | |||
Table data modified[5] |
Note new HUGO Gene Nomenclature Committee (HGNC)
- Male sex - SF-1 (NR5A1) Nuclear Receptor Subfamily 5, Group A, Member 1
- Female sex - DAX-1 (NR0B1) Nuclear Receptor Subfamily 0, Group B, Member 1
Stages
- Development of the indifferent gonad - (genital ridge) early embryo
- Differentiation of gonad - (testis or ovary) late embryo, defining event in sexual differentiation
- Differentiation of internal genital organs and ducts - late embryo to fetal
- Differentiation of external genitalia - fetal
- Development of secondary sexual characteristics - puberty
Male – Sertoli Cells
- Human SRY activation not known
- Mouse SRY activation - Wilms tumor 1 (Wt1), GATA binding protein 4 (Gata4), zinc fnger protein, fog family member 2 (Zfpm2), chromobox homolog 2 (Cbx2), mitogen-activated protein kinase 4 (Map3k4), insulin receptors.
- SRY activates key transcription factor SOX9 in Sertoli cells
- SOX9 requires positive regulatory loop
- Sox9 and FGF9 feed-forward loop upregulates Fgf9 expression
- repression of a WNT4
- Retinoic acid (RA) - required later for Sertoli to germ cells essential for adult mammalian spermatogenesis.
Human SOX Family | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Female - Granulosa Cells
- Wnt4 (1p36.12) secreted protein, inhibit testis-specific processes
- mesonephros migration of endothelial cells
- repress steroidogenesis by Sf1 or blocking recruitment of steroidogenic cell precursors
- RSPO1 (1p34.3) secreted protein
- agonist WNT4 signalling
- FOXL2 (3q22.3) transcription factor
- repress Sox9 by synergistic interaction with estrogen receptors α and -β (ER-α-β)
- postnatal follicle development and female fertility maintenance
- FOXL2 gene are associated with syndromic and non-syndromic ovarian failure.
- Mutations in FOXL2 result in blepharophimosis/ptosis/epicanthus inversus syndrome (BPES).
Human WNT Family | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Gonad Differentiation
Animations summarising the early morphological development of the male and female gonad.
Male | Female |
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<html5media height="600" width="300">File:Testis 001.mp4</html5media> | <html5media height="600" width="300">File:Ovary_001.mp4</html5media> |
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Testis
Testis Pre-Puberty | Testis Post-Puberty |
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Ovary
Ovary Pre-Puberty
Internal Genital
Male
Human Embryo (GA week 10, stage 22) pelvic level cross-section. | |
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G5 urogenital | urogenital |
peritoneal cavity | rete tesits | G3 testis |
- week 8 - males Sertoli cells secrete anti müllerian hormone (AMH), which causes regression of the paramesonephric ducts between the 8th and 10th weeks.
- week 9 to 10 - gonadal cells begin to produce testosterone, which maintains the mesonephric ducts.
- mesonephric ducts go on to form the internal genital tract:
- rete testis
- ductuli efferentes
- vas deferens
Trigone - note trigone does not include mesonephric contribution, though the ducts do shift location to open into teh male urethra.
<html5media height="380" width="450">File:Trigone_001.mp4</html5media>
Female
Mouse paramesonephric duct (Müllerian duct)[7] |
This mouse image shows the relationship between the mesonephric and paramesonephric ducts opening into the urogenital sinus.
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Female Uterus and Vagina (between GA week 11 and 22) | |
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<html5media height="500" width="490">File:Uterus_002.mp4</html5media> | Note - the entire vagina is formed from the paramesonephric (Müllerian) duct and does not have a contribution from the urogenital endoderm.
The uterus and broad ligament will eventulaly divide the pelvic cavity into two separate pouches.
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External Genital
The unisex genital tubercle (GA week 12) is a major contributor to both sexes external genitalia.
Male | Female |
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<html5media height="380" width="270">File:Male_external_001.mp4</html5media> | <html5media height="380" width="270">File:Female_external_001.mp4</html5media> |
Animation showing the development of external male genitalia from the indifferent external structure ((GA week 11 to 14 approximately).
The reduction of Testosterone to active metabolite, 5α-dihydrotestosterone (DHT) is carried out by the enzyme 5α-reductase expressed in the region or male external genitaila and prostate gland. Note that there are several 5α-reductase isoforms that differ in both tissue distribution and kinetics.
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Animation showing the development of external female genitalia from the indifferent external structure (GA week 11 to 14 approximately).
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Testosterone metabolism
Biochemical pathway showing testosterone metabolism, to a more active form, then inactivation and conjugation for elimination.
5-alpha reductase - enzyme that convert testosterone into the more potent dihydrotestosterone (DHT).
Testes Descent
<html5media height="400" width="600">File:Testis_Descent_001.mp4</html5media> | The animation shows the descent of the testes (between GA week 9 to 38, birth).
Descent of the testes into the scrotal sac begins generally during week 26 and may take several days.
Incomplete or failed descent can occur unilaterally or bilaterally, is more common in premature births, and can be completed postnatally. (see also cryptorchidism). |
Data from a study of male human fetal (between 10 and 35 weeks) gonad position[8]
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Human Embryo Development
These sagittal MRI scans of GA week 8-10 show overall embryo development during this period.
Week 8 | Week 10 |
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<html5media height="500" width="400">File:Stage16 MRI S01.mp4</html5media> | <html5media height="600" width="500">File:Stage23 MRI S02.mp4</html5media> |
Kidneys
<html5media height="400" width="500">File:Nephron_development.mp4</html5media>This animation shows the process of early renal (kidney) development.
Legend
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The morphological events and localization of renal progenitors occurring during nephron development in the adult human kidney.[9]
Mesenchymal cells near the tips of the branching ureteric bud undergo epithelial transition and differentiate through a series of forms made up of renal progenitors (red)
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After nephron development has completed and concomitant with the development of the renal papilla in the newborn, the thin ascending limb of Henle’s loops is generated as an outgrowth from the S3 segment of the proximal tubule and from the distal tubule anlage of the nephron. (birds and mammals) |
Adrenals
- Week 6 - fetal cortex, from mesothelium adjacent to dorsal mesentery; Medulla, neural crest cells from adjacent sympathetic ganglia
- Fetal Adrenals - fetal cortex later replaced by adult cortex
- Adult cortex - mesothelium mesenchyme encloses fetal cortex
Adrenal Cortex
- Late Fetal Period - differentiates to form cortical zones
- Birth - zona glomerulosa, zona fasiculata present
- Year 3 - zona reticularis present
Adrenal Medulla
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Adrenal gland development and steroid hormone synthesis | |||||||||||||||
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Overview cartoon of changes in adrenal gland structure and steroid hormone syntesis[10]
HypothalamusHypothalamus is a neuroendocrine organ linking the brain to the endocrine system. There are also hypothalamic cells that hormone-responsive during development and in the adult. Recently there has been shift in describing neurological development from the traditional morphological model of “primary and secondary vesicles” with developmental origin and gene expression model “prosomeric development”
Hormones - Corticotrophin releasing hormone (CRH), Thyrotrophin releasing hormone (TRH), Arginine vasopressin (AVP), Gonadotrophin releasing hormone (GnRH), Growth hormone releasing hormone (GHRH), Somatostatin, Prolactin relasing factor (PRF), Dopamine Pituitary
Neurohypophysis
Pituitary Timeline
Thyroid
Thyroid Timeline
Human thyroid system and neural development Parathyroid - different embryonic origin
BreastPuberty ChangesThe Tanner scale is an anatomical staging system for measuring male/female sexual development at puberty. Stages were based upon genital and secondary sex characteristic development and named after James M. Tanner who, along with W.A. Marshall, published the stages for both girls (1969 [12]) and boys (1970 [13]). It is not a system for determining age. (More? Puberty Development | Tanner stages) References
Cite this page: Hill, M.A. (2024, June 3) Embryology Royal Hospital for Women - Reproductive Medicine Seminar 2018. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Royal_Hospital_for_Women_-_Reproductive_Medicine_Seminar_2018
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