Medaka Development: Difference between revisions

From Embryology
Line 22: Line 22:
===Developmental Stages===
===Developmental Stages===


Stage 0. Unfertilized eggs
{|
 
|-
Stage 1 (3 min). Activated egg stage
| Stage
 
| Time
Stage 2. Blastodisc stage
| Event
2.4. Stage 3 (1 h 5 min). 2 cell stage
|-
2.5. Stage 4 (1 h 45 min). 4 cell stage
|  0
2.6. Stage 5 (2 h 20 min). 8 cell stage
|
2.7. Stage 6 (2 h 55 min). 16 cell stage
| Unfertilized eggs
2.8. Stage 7 (3 h 30 min). 32 cell stage
|-
2.9. Stage 8 (4 h 5 min). Early morula stage
|  1 | 3 min | Activated egg
2.10. Stage 9 (5 h 15 min). Late morula stage
|-
2.11. Stage 10 (6 h 30 min). Early blastula stage
|  2. Blastodisc
2.12. Stage 11 (8 h 15 min). Late blastula stage
|-
2.13. Stage 12 (10 h 20 min). Pre-early gastrula stage
|  3 | 1 h 5 min | 2 cell
2.14. Stage 13 (13 h). Early gastrula stage
|-
2.15. Stage 14 (15 h). Pre-mid-gastrula stage
|  4 | 1 h 45 min | 4 cell
2.16. Stage 15 (17 h 30 min). Mid-gastrula stage
|-
2.17. Stage 16 (21 h). Late gastrula stage
|  5 | 2 h 20 min | 8 cell
2.18. Stage 17 (1 day 1 h). Early neurula stage (head formation)
|-
2.19. Stage 18 (1 day 2 h). Late neurula stage (optic bud formation)
|  6 | 2 h 55 min | 16 cell
2.20. Stage 19 (1 day 3 h 30 min). 2 somite stage
|-
2.21. Stage 20 (1 day 7 h 30 min). 4 somite stage
|  7 | 3 h 30 min | 32 cell
2.22. Stage 21 (1 day 10 h). 6 somite stage (brain regionalization and otic vesicle formation)
|-
2.23. Stage 22 (1 day 14 h). 9 somite stage (appearance of heart anlage)
|  8 | 4 h 5 min | Early morula
2.24. Stage 23 (1 day 17 h). 12 somite stage (formation of tubular heart)
|-
2.25. Stage 24 (1 day 20 h). 16 somite stage (start of heart beating)
|  9 | 5 h 15 min | Late morula
2.26. Stage 25 (2 days 2 h). 18–19 somite stage (onset of blood circulation)
|-
2.27. Stage 26 (2 days 6 h). 22 somite stage (development of guanophores and vacuolization of the notochord)
|  10 | 6 h 30 min | Early blastula
2.28. Stage 27 (2 days 10 h). 24 somite stage (appearance of pectoral fin bud)
|-
2.29. Stage 28 (2 days 16 h). 30 somite stage (onset of retinal pigmentation)
|  11 | 8 h 15 min | Late blastula
2.30. Stage 29 (3 days 2 h). 34 somite stage (internal ear formation)
|-
2.31. Stage 30 (3 days 10 h). 35 somite stage (blood vessel development)
|  12 | 10 h 20 min | Pre-early gastrula
2.32. Stage 31 (3 days 23 h). Gill blood vessel formation stage
|-
2.33. Stage 32 (4 days 5 h). Somite completion stage (formation of pronephros and air bladder)
|  13 | 13 h | Early gastrula
2.34. Stage 33 (4 days 10 h). Stage at which notochord vacuolization is completed
|-
2.35. Stage 34 (5 days 1 h). Pectoral fin blood circulation stage
|  14 | 15 h | Pre-mid-gastrula
2.36. Stage 35 (5 days 12 h). Stage at which visceral blood vessels form
|-
2.37. Stage 36 (6 days). Heart development stage
|  15 | 17 h 30 min | Mid-gastrula
2.38. Stage 37 (7 days). Pericardial cavity formation stage
|-
2.39. Stage 38 (8 days). Spleen development stage (differentiation of caudal fin begins)
|  16 | 21 h | Late gastrula  
2.40. Stage 39 (9 days). Hatching stage
|-
2.41. Stage 40. 1st fry stage
|  17 | 1 day 1 h | Early neurula (head formation)
2.42. Stage 41
|-
2.43. Stage 42
|  18 | 1 day 2 h | Late neurula (optic bud formation)
2.44. Stage 43
|-
2.45. Stage 44
|  19 | 1 day 3 h 30 min | 2 somite
2.46. Stage 45
|-
|  20 | 1 day 7 h 30 min | 4 somite
|-
|  21 | 1 day 10 h | 6 somite |  (brain regionalization and otic vesicle formation)
|-
|  22 | 1 day 14 h | 9 somite |  (appearance of heart anlage)
|-
|  23 | 1 day 17 h | 12 somite |  (formation of tubular heart)
|-
|  24 | 1 day 20 h | 16 somite |  (start of heart beating)
|-
|  25 | 2 days 2 h | 18–19 somite |  (onset of blood circulation)
|-
|  26 | 2 days 6 h | 22 somite |  (development of guanophores and vacuolization of the notochord)
|-
|  27 | 2 days 10 h | 24 somite |  (appearance of pectoral fin bud)
|-
|  28 | 2 days 16 h | 30 somite |  (onset of retinal pigmentation)
|-
|  29 | 3 days 2 h | 34 somite |  (internal ear formation)
|-
|  30 | 3 days 10 h | 35 somite |  (blood vessel development)
|-
|  31 | 3 days 23 h | Gill blood vessel formation
|-
|  32 | 4 days 5 h | Somite completion |  (formation of pronephros and air bladder)
|-
|  33 | 4 days 10 h | |  at which notochord vacuolization is completed
|-
|  34 | 5 days 1 h | Pectoral fin blood circulation
|-
|  35 | 5 days 12 h | |  at which visceral blood vessels form
|-
|  36 | 6 days | Heart development
|-
|  37 | 7 days | Pericardial cavity formation
|-
|  38 | 8 days | Spleen development |  (differentiation of caudal fin begins)
|-
|  39 | 9 days | Hatching
|-
|  40 | 1st fry |
|-
|  41 | |
|-
|  42 | |
|-
|  43 | |
|-
|  44 | |
|-
|  45 | |
|}


==References==
==References==

Revision as of 16:16, 14 July 2010

Introduction

Medaka

Medaka Oryzias latipes or Japanese rice fish is a member of the killifish family first described in 1846 and has been widely used as a aquarium fish. A modified aquarium version with a genetically modified fluorescent (GFP) version also now available in some countries.

A recent study by Iwamatsu T., 2004[1] has characterised the stages of normal fish development.

Medaka fish were also the first for the first vertebrate animal to mate in space (The International Microgravity Laboratory IML-2/STS-65 mission in 1994) as a developmental model for space experiments. The fish has also been used in studies of pigmentation development.

Links: original Medaka page

Taxon

cellular organisms; Eukaryota; Fungi/Metazoa group; Metazoa; Eumetazoa; Bilateria; Coelomata; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Elopocephala; Clupeocephala; Euteleostei; Neognathi; Neoteleostei; Eurypterygii; Ctenosquamata; Acanthomorpha; Euacanthomorpha; Holacanthopterygii; Acanthopterygii; Euacanthopterygii; Percomorpha; Smegmamorpha; Atherinomorpha; Beloniformes; Adrianichthyoidei; Adrianichthyidae; Oryziinae; Oryzias

Development Overview

Development has been characerised by light microscope observation into 39 prehatch stages and 6 posthatch stages.

Prehatch features observed included: number and size of blastomeres, form of the blastoderm, extent of epiboly, central nervous system, number and form of somites, optic and otic, notochord, heart, blood circulation, the size and movement of the body, tail, membranous fin (fin fold), viscera (liver gallbladder, gut tube), spleen and swim (air) bladder.

Posthatch features observed included: fins, scales and secondary sexual characteristics.

Developmental Stages

Stage Time Event
0 Unfertilized eggs
3 min | Activated egg
2. Blastodisc
1 h 5 min | 2 cell
1 h 45 min | 4 cell
2 h 20 min | 8 cell
2 h 55 min | 16 cell
3 h 30 min | 32 cell
4 h 5 min | Early morula
5 h 15 min | Late morula
6 h 30 min | Early blastula
8 h 15 min | Late blastula
10 h 20 min | Pre-early gastrula
13 h | Early gastrula
15 h | Pre-mid-gastrula
17 h 30 min | Mid-gastrula
21 h | Late gastrula
1 day 1 h | Early neurula (head formation)
1 day 2 h | Late neurula (optic bud formation)
1 day 3 h 30 min | 2 somite
1 day 7 h 30 min | 4 somite
1 day 10 h | 6 somite | (brain regionalization and otic vesicle formation)
1 day 14 h | 9 somite | (appearance of heart anlage)
1 day 17 h | 12 somite | (formation of tubular heart)
1 day 20 h | 16 somite | (start of heart beating)
2 days 2 h | 18–19 somite | (onset of blood circulation)
2 days 6 h | 22 somite | (development of guanophores and vacuolization of the notochord)
2 days 10 h | 24 somite | (appearance of pectoral fin bud)
2 days 16 h | 30 somite | (onset of retinal pigmentation)
3 days 2 h | 34 somite | (internal ear formation)
3 days 10 h | 35 somite | (blood vessel development)
3 days 23 h | Gill blood vessel formation
4 days 5 h | Somite completion | (formation of pronephros and air bladder)
4 days 10 h | | at which notochord vacuolization is completed
5 days 1 h | Pectoral fin blood circulation
5 days 12 h | | at which visceral blood vessels form
6 days | Heart development
7 days | Pericardial cavity formation
8 days | Spleen development | (differentiation of caudal fin begins)
9 days | Hatching
1st fry |
|
|
|
|
|

References

  1. ↑ <pubmed>15210170</pubmed>


Search Pubmed

Search Pubmed: Medaka Development


Animal Development: axolotl | bat | cat | chicken | cow | dog | dolphin | echidna | fly | frog | goat | grasshopper | guinea pig | hamster | horse | kangaroo | koala | lizard | medaka | mouse | opossum | pig | platypus | rabbit | rat | salamander | sea squirt | sea urchin | sheep | worm | zebrafish | life cycles | development timetable | development models | K12
Historic Embryology  
1897 Pig | 1900 Chicken | 1901 Lungfish | 1904 Sand Lizard | 1905 Rabbit | 1906 Deer | 1907 Tarsiers | 1908 Human | 1909 Northern Lapwing | 1909 South American and African Lungfish | 1910 Salamander | 1951 Frog | Embryology History | Historic Disclaimer

Glossary Links

Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link

Cite this page: Hill, M.A. (2024, June 3) Embryology Medaka Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Medaka_Development

What Links Here?
© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
Retrieved from ‘https://embryology.med.unsw.edu.au/embryology/index.php?title=Medaka_Development&oldid=27303’
Powered by MediaWiki