Mouse Lipid Droplets Movie: Difference between revisions
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===Mouse Embryo Lipid Droplets== | ===Mouse Embryo Lipid Droplets=== | ||
[[File: | [[File:Mouse_lipid_droplets_icon.jpg|100px|right]] | ||
Time-lapse of embryo development | Time-lapse of embryo development optical sections of the mouse embryo. | ||
* At fertilization, lipid droplets are small and uniformly distributed. | |||
* Implanting blastocyst lipid droplets are larger and enriched in the invading giant cells of the trophectoderm. | |||
* Movie starts at the compacted morula stage and development is followed till the blastocyst stage. | |||
* The majority of third harmonic generation (THG) signal is generated by lipid droplets, which become fewer and larger over the course of development. | |||
* Imaged with third harmonic generation (THG, grey) and second harmonic generation (SHG magenta). | |||
* Images were captured at 20 minute intervals. Scale = 20 μm. | |||
'''Harmonic Generation Microscopy''' (HGM) | |||
* laser scanning microscope three-dimensional, label-free imaging of developing embryos | |||
* takes advantage of second and third harmonic generation (SHG and THG respectively) | |||
* result in the generation of photons of half and one-third the illumination wavelength respectively. | |||
:'''Links:''' [[:Media:Mouse lipid droplets.mp4|MP4 version]] | [[Mouse Development]] | |||
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Latest revision as of 22:56, 17 June 2014
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<html5media height="580" width="465">File:Mouse lipid droplets.mp4</html5media> |
Mouse Embryo Lipid DropletsTime-lapse of embryo development optical sections of the mouse embryo.
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Reference
<pubmed>20525231</pubmed>| PMC3238212 | BMC Cell Biol.
Copyright
© 2010 Watanabe et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Cite this page: Hill, M.A. (2024, June 2) Embryology Mouse Lipid Droplets Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Lipid_Droplets_Movie
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G