File:Mouse Lmx1b gene expression.jpg: Difference between revisions
No edit summary |
No edit summary |
||
(One intermediate revision by the same user not shown) | |||
Line 1: | Line 1: | ||
==Mouse Lmx1b Gene Expression== | ==Mouse Lmx1b Gene Expression== | ||
Lmx1b expressed in the dorsal mesenchyme (b). | Lmx1b LIM homeobox transcription factor 1 beta (Lmx1b) expressed in the dorsal mesenchyme (b). | ||
Lmx1b: [[#Detection of genes regulated by Lmx1b during limb dorsalization|limb dorsalization]] | [http://www.ncbi.nlm.nih.gov/gene?db=gene&cmd=Retrieve&dopt=full_report&list_uids=16917 Gene] | PMID 22417325 | |||
Line 7: | Line 11: | ||
Figure 3. doi:10.1371/journal.pone.0028358.g003 | Figure 3. doi:10.1371/journal.pone.0028358.g003 | ||
---- | |||
===Detection of genes regulated by Lmx1b during limb dorsalization=== | |||
Dev Growth Differ. 2012 May;54(4):451-62. doi: 10.1111/j.1440-169X.2012.01331.x. Epub 2012 Mar 15. | |||
Feenstra JM, Kanaya K, Pira CU, Hoffman SE, Eppey RJ, Oberg KC. | |||
Source | |||
Division of Human Anatomy, Department of Pathology and Human Anatomy, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA. | |||
Abstract | |||
Lmx1b is a homeodomain transcription factor that regulates dorsal identity during limb development. Lmx1b knockout (KO) mice develop distal ventral-ventral limbs. Although induction of Lmx1b is linked to Wnt7a expression in the dorsal limb ectoderm, the downstream targets of Lmx1b that accomplish limb dorsalization are unknown. To identify genes targeted by Lmx1b, we compared gene arrays from Lmx1b KO and wild type mouse limbs during limb dorsalization, i.e., 11.5, 12.5, and 13.5 days post coitum. We identified 54 target genes that were differentially expressed in all three stages. Several skeletal targets, including Emx2, Matrilin1 and Matrilin4, demonstrated a loss of scapular expression in the Lmx1b KO mice, supporting a role for Lmx1b in scapula development. Furthermore, the relative abundance of extracellular matrix-related soft tissue targets regulated by Lmx1b, such as collagens and proteoglycans, suggests a mechanism that includes changes in the extracellular matrix composition to accomplish limb dorsalization. Our study provides the most comprehensive characterization of genes regulated by Lmx1b during limb development to-date and provides targets for further investigation. | |||
© 2012 The Authors. Development, Growth & Differentiation © 2012 Japanese Society of Developmental Biologists. | |||
PMID 22417325 | |||
[[Category:Mouse E10.5]] [[Category:Mouse E11.5]] [[Category:Mouse E12.5]] | [[Category:Mouse E10.5]] [[Category:Mouse E11.5]] [[Category:Mouse E12.5]] |
Latest revision as of 00:41, 14 February 2013
Mouse Lmx1b Gene Expression
Lmx1b LIM homeobox transcription factor 1 beta (Lmx1b) expressed in the dorsal mesenchyme (b).
Lmx1b: limb dorsalization | Gene | PMID 22417325
- Limb Links: Mouse limb skeleton cartoon | Mouse limb tissue development | Limb Development | Mouse Development
Reference
Taher L, Collette NM, Murugesh D, Maxwell E, Ovcharenko I & Loots GG. (2011). Global gene expression analysis of murine limb development. PLoS ONE , 6, e28358. PMID: 22174793 DOI.
Copyright
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Figure 3. doi:10.1371/journal.pone.0028358.g003
Detection of genes regulated by Lmx1b during limb dorsalization
Dev Growth Differ. 2012 May;54(4):451-62. doi: 10.1111/j.1440-169X.2012.01331.x. Epub 2012 Mar 15.
Feenstra JM, Kanaya K, Pira CU, Hoffman SE, Eppey RJ, Oberg KC. Source Division of Human Anatomy, Department of Pathology and Human Anatomy, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.
Abstract
Lmx1b is a homeodomain transcription factor that regulates dorsal identity during limb development. Lmx1b knockout (KO) mice develop distal ventral-ventral limbs. Although induction of Lmx1b is linked to Wnt7a expression in the dorsal limb ectoderm, the downstream targets of Lmx1b that accomplish limb dorsalization are unknown. To identify genes targeted by Lmx1b, we compared gene arrays from Lmx1b KO and wild type mouse limbs during limb dorsalization, i.e., 11.5, 12.5, and 13.5 days post coitum. We identified 54 target genes that were differentially expressed in all three stages. Several skeletal targets, including Emx2, Matrilin1 and Matrilin4, demonstrated a loss of scapular expression in the Lmx1b KO mice, supporting a role for Lmx1b in scapula development. Furthermore, the relative abundance of extracellular matrix-related soft tissue targets regulated by Lmx1b, such as collagens and proteoglycans, suggests a mechanism that includes changes in the extracellular matrix composition to accomplish limb dorsalization. Our study provides the most comprehensive characterization of genes regulated by Lmx1b during limb development to-date and provides targets for further investigation. © 2012 The Authors. Development, Growth & Differentiation © 2012 Japanese Society of Developmental Biologists.
PMID 22417325
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 00:33, 14 February 2013 | 1,624 × 550 (104 KB) | Z8600021 (talk | contribs) | ==Mouse Lmx1b Gene Expression== Lmx1b expressed in the dorsal mesenchyme (b). {{Mouse_limb_links}} Figure 3. doi:10.1371/journal.pone.0028358.g003 Category:Mouse E13.5 |
You cannot overwrite this file.
File usage
There are no pages that use this file.