File:Mouse thymus development 02.jpg

Expression of Transcription Factors in the pharyngeal region at E10.5

3D reconstructions of the 3rd pouch reveal differentially regionalized expression patterns of Nkx2-5, Nkx2-6, Isl1, Gata3, Foxg1 and Sox2 at E10.5 days.

(A–C) Expression pattern of Gcm2 in the third pouch at E10.5.

Whole mount (A) and parasagittal sections (B) of E10.5 embryos hybridized with a Gcm2 probe.

(C) A left third pouch was reconstructed showing Gcm2 expression in red.

(D–I) 3D reconstructions of the left 3rd pouch showing the expression of each gene in blue. Orientation of the reconstructed pouch is shown in (D).

D, dorsal; V, ventral; A, anterior; P, posterior; L, lateral; M, medial. Scale bar represents 100 µm.

Note - In the E11.5 third pouch endoderm, the thymus domain can be identified by the expression of Foxn1 in the ventral posterior portion of the pouch, complimentary to the parathyroid domain which is marked by Gcm2 expression.

Reference

Wei Q & Condie BG. (2011). A focused in situ hybridization screen identifies candidate transcriptional regulators of thymic epithelial cell development and function. PLoS ONE , 6, e26795. PMID: 22087235 DOI.

Copyright

© 2011 Wei, Condie. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Original file name: Figure 3. Journal.pone.0026795.g003.png

doi:10.1371/journal.pone.0026795.g003

Mouse thymus development 01.jpg

Cite this page: Hill, M.A. (2024, April 19) Embryology Mouse thymus development 02.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Mouse_thymus_development_02.jpg

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