File:Mouse- forelimb bud Shh expression and BMP pathway.jpg

Shh expression and BMP pathway activity in forelimb buds

Excerpt from Figure 6. showing only the normal limb gene expression.

(A) No Shh expression is detected in the posterior mesenchyme of H2Δ/Δc and H2Δ/ΔcGli3Xt/Xt limb buds at E10.25 (32–33 somites).

(B) Bmp4, Grem1, and Msx2 expression at E10.5 (34–35 somites). Note that Grem1 expression is activated, but not up-regulated and expanded distal-anterior in H2Δ/Δc limb buds. In contrast, the Grem1 expression domain appears rather uniform in the majority of all H2Δ/ΔcGli3Xt/Xt limb buds.

(C) Q–PCR quantitation of Shh, Bmp4, Grem1 and Msx2 expression in single limb buds of mouse embryos of the indicated genotypes at ~E10.5 (34–37 somites). Boxes show the average (± standard deviation), dots indicate levels in individual limb bud determined by triplicate analysis. The vertical axis indicates expression levels in percentages of wild-type levels (wild-type average set at 100%). Wt: wild-type (n = 8 single limb buds analyzed); H2: H2Δ/Δc (n = 8); Gli3: Gli3Xt/Xt (n = 7); H2Gli3: H2Δ/ΔcGli3Xt/Xt (n = 6).

All differences discussed in the text are statistically highly significant (p-values between p<0.001 and p<0.05 using Mann-Whitney tests).

Mouse Limb Images: Tbx3 and Tbx2 forelimb E10 | Alx3 and Gli3 forelimb E10 | Fgf and Hox forelimb E10.5 | Bmp4 forelimb E11.5 | Bmp4 hindlimb E11.5 | Shh forelimb E11.5 | Fgf8 hindlimb E11.5 | Sox9 forelimb E12.5 | Msx2 forelimb E12.5 | Shh hindlimb E12.5

Links: Fgf | Hox | Shh | Sox | Limb Development | Mouse Development

Reference

<pubmed>20386744</pubmed>| PMC2851570 | PLoS Genet.

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Original File name: journal.pgen.1000901.g003.png