File:Limb changing 3D geometry.jpg

The changing 3D geometry during limb development

(A) The right hind limb bud at stage E11.0 from the freshly dissected embryo, and (B) a right hind limb bud from another embryo at stage E11.25, 6 h older than (A).

(C) The OPT scans were converted into an iso-surface, a 3D contour of the embryo.

The limb bud shapes St0, (D) and St1 (E) were virtually dissected from the whole-embryo iso-surface.

(F) A comparison between the two empirically measured shapes St0 (white) and St1 (blue) which highlights the shape change over 6 h of development. The main axes are shown anterio-posterior (AP), dorso-ventral (DV), and proximo-distal (PD).

(G) A virtual slice through the fully tetrahedtralised St0 mesh. The surface is discretised with a triangular mesh (green), and the internal mesenchymal volume is discretised with a 3D tetrahedral mesh (red).

Original file name: Figure 2. Journal.pbio.1000420.g002.png (resized using photoshop and converted to jpg)

Reference

<pubmed>20644711</pubmed>| PMC2903592 | PLoS

Citation: Boehm B, Westerberg H, Lesnicar-Pucko G, Raja S, Rautschka M, et al. (2010) The Role of Spatially Controlled Cell Proliferation in Limb Bud Morphogenesis. PLoS Biol 8(7): e1000420. doi:10.1371/journal.pbio.1000420

Copyright: © 2010 Boehm et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.