File:Human spermatozoa phospholipase C zeta.jpg

Human Spermatozoa Phospholipase C zeta Localization

Developmental localization of PLCζ in human (B, C) testes utilizing anti-hmPLCζ antibody. In human, there are six stages (I–VI) of the cycle of the seminiferous epithelium.

PLCζ is compartmentalized as part of the acrosome early in human spermiogenesis and is secreted during sperm maturation in the epididymis. No evidence was found that PLCζ is incorporated into the detergent-resistant perinuclear theca fraction where sperm-borne oocyte activating factor (SOAF) resides.

• B - (stage II) PLCζ accumulates over the acrosomic vesicle (AV) of step 2 spermatids. Little immunoreactivity is found elsewhere in the epithelium and the elongated spermatids (arrowheads) appear unreactive.
• C - (stage IV) step 4 spermatids the fully formed acrosome is intensely labeled (arrows).

Bars = 20 µm

Reference

<pubmed>22428063</pubmed>| PLoS One.

Citation: Aarabi M, Yu Y, Xu W, Tse MY, Pang SC, et al. (2012) The Testicular and Epididymal Expression Profile of PLCζ in Mouse and Human Does Not Support Its Role as a Sperm-Borne Oocyte Activating Factor. PLoS ONE 7(3): e33496. doi:10.1371/journal.pone.0033496

Copyright: © 2012 Aarabi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Panel B and C from Figure 2. Developmental localization of PLCζ in mouse (A) and human (B, C) testes utilizing anti-hmPLCζ antibody.