File:Human spermatozoa acrosomal protein SP-10.jpg

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Human Spermatozoa Acrosomal Protein SP-10

Immunofluorescent/differential interference contrast (DIC) micrographs (A–H) and diagrammatic representations (a–h; modified from Holstein and Roosen-Runge, 1981) of enzyme-dissociated human testicular cells.

  • A - This early round spermatid demonstrates a small acrosomal vesicle (overlying the nucleus) stained with the acrosomal marker SP-10 (green). SPANXa/d staining is associated with the nuclear periphery.
  • B - Early cap-phase spermatid showing SPANXa/d staining of the nuclear periphery posterior to the early acrosomal cap. As the acrosomal cap migrates posteriorly and the spermatid begins to elongate
  • C–F - SPANXa/d staining is associated with the nuclear envelope posterior to the acrosomal cap.
  • D and E - evident that a SPANXa/d-negative region intervenes between the equatorial bulbs on either side of the acrosome and the SPANXa/d-positive nuclear envelope.
  • G - In elongated spermatids
  • H - testicular spermatozoa SPANXa/d staining is seen in the posterior head and cytoplasmic droplet.

Occasional nuclear vacuoles demonstrating SPANXa/d staining are observed (B and C, arrows). × 1250

Legend
  • A - acrosome
  • An - annulus
  • C - centriole
  • Fs - flagellum
  • M - mitochondria
  • Mt - manchette
  • N - nucleus
  • Sb - spindle-shaped body

Labels

  • blue - DNA (label - 4′6-diamidino-2-phenylindole, DAPl)
  • red - acrosomal protein SP-10 (label - Tetramethylrhodamine B isothiocyanate, TRITC)
  • green - SPANXa/d. (label - Fluorescein isothiocyanate, FITC)


Links: Spermatozoa Development


Reference

<pubmed>17012309</pubmed>| Mol Hum Reprod.

Copyright

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org


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Only Figure 6. from the original article is displayed here.

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