File:Human lung pseudoglandular.jpg

Human Lung Pseudoglandular

Immunohistochemical stainings for E- and N-cadherin and β-catenin during pseudoglandular period of developing human lung.

1A and 1B. Epithelium of the developing airways is positive for E-cadherin (arrows).

1C and 1 D. Epithelium of some developing airways is positive for N-cadherin (arrows) whereas other developing airways are negative for N-cadherin (short arrows).

1E and 1F. Epithelial and mesenchymal cells of developing airways are positive for β-catenin. In most airways epithelial cells display nuclear positivity (arrows) whereas some airways exhibit membrane-bound positivity (short arrows).

1G. A high power field image showing nuclear positivity for β-catenin in epithelial cells of a small developing airway (arrows) surrounded by positively stained mesenchymal cells (short arrows). 1

H. A negative control in which the primary antibody has been substituted with non-immune serum. Arrows are indicating developing airways.

Scale bars = 40 μm in 1A, 1C, 1F and 1H; scale bars = 20 μm in 1B, 1 D and 1G, and scale bar = 80 μm in 1E.

The expressions of protein and mRNAs of E- and N-cadherin and β-catenin were higher in early gestation compared to of the end.

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Original file name: 1471-213X-10-113-1.jpg

Reference

Kaarteenaho R, Lappi-Blanco E, Lehtonen S. Epithelial N-cadherin and nuclear β-catenin are up-regulated during early development of human lung. BMC Dev Biol. 2010 Nov 16;10:113. PMID: 21080917 | PMC2995473 | BMC Dev Biol.

Kaarteenaho et al. BMC Developmental Biology 2010 10:113 doi:10.1186/1471-213X-10-113

© 2010 Kaarteenaho et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.