File:Bovine blastocyst KRT18 and MYL6 expression.jpg

Bovine blastocyst (in vitro) KRT18 and MYL6 expression

A) Image of the surface of a bovine blastocyst day 8 p.i.labelled for KRT18. The nuclei were stained with propidium iodide. KRT18 was expressed at the cell-cell contact sites of the trophectoderm cells.

B) Image of a midplane section through a bovine blastocyst day 8 p.i. labelled with antibodies for MYL6. The nuclei were stained with propidium iodide. MYL6 proteins were located in the trophectoderm cells surrounding the blastocoel cavity.

original file name: Figure 3. 1471-213X-7-64-3.jpg http://www.biomedcentral.com/1471-213X/7/64/figure/F3

"By subtractive cDNA cloning, candidate genes involved in blastocyst formation were identified. For several candidate genes, important differences in gene expression were observed between in vivo and in vitro produced embryos, reflecting the influence of the in vitro culture system on the embryonic gene expression. Both RNA and protein expression analysis demonstrated that KRT18, FN1 and MYL6 are differentially expressed during preimplantation embryo development and those genes can be considered as markers for bovine blastocyst formation."

Reference

<pubmed>17559642</pubmed>| BMC Dev Biol.

Goossens et al. BMC Developmental Biology 2007 7:64 doi:10.1186/1471-213X-7-64

© 2007 Goossens et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.