UNSW Embryology

UNSW Anatomy Histology Slides

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Other Pages: UNSW Slides by SystemWWW Histology by System

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Introduction The table below lists the slide sets used in teaching Histology to undergraduate Medical and Science Students in the School of Anatomy. The most current catalogue of slides can also be found at the School of Medical Sciences WWW Site . Note that the overall composition of slide sets will change with time. These slides are used to show both developing and adult tissues using typical histological stains. Additional demonstration slides are used in classes. Slides should be viewed in relation to the types of tissues that are developing in the embryo. About View Slides In the table below clicking on the View Slides option will open an unlabelled PDF image of the listed Histology slide at different magnifications (Microscope magnification differs from those in Lab). Use your web browser option to "open link in new window" to keep this page and table open. These images have security that prevents their alteration or printing and were originally prepared as a project by 2 medical students ( Immie and Kevin) to help with your histology revision. Histology in Notes Section On page 5 of each section of the Notes is a list of relevant UNSW slides and links to external sites relevant to that system. No attempt has been made in this current Program to give full histological descriptions of slides. I have also included links to the start pages of some good external WWW Histology resources which have histological images and quiz questions. In addition, there is a system by system break down of these external WWW resources also available. The Fabric of Life The School of Anatomy UNSW has an excellent Histology Program under development "The Fabric of Life", for more details contact Dr B. Freeman. This program is currently only available from computers in the Department of Anatomy or the Biomedical Library.

UNSW HISTOLOGY SLIDES

No.	Tissue	Animal	Stain	View Slides (magnification)
1	Umbilical cord	human	H & E
1A	Umbilical cord	human	Gomori Trichrome
2	Lymph node (reticular fibres)	cat	Silver, Fast Green
3	Mitosis	onion root	Iron Haematoxylin
4	Mesentery (whole mount)	rat	Aldehyde Pararosanilin, H & Fast Green	10 20 40
4A	Phagocytosis (liver-ferritin;adipose tissue-carbon)	rat	Perls' Reaction, Nuclear Fast Red, Tartrazine	2 40
5	Cartilage (epiglottis)	dog	Verhoeff's elastic, Tartrazine	5 10 20 40
5A	Fibrocartilage (I.V. disc)	human	H & E	10 10 10 20 20
6	Lung (reticular fibres)	cat	Silver, Nuclear Fast Red,Tartrazine
6A	Lung (elastic fibres7	guinea pig	Aldehyde Pararosanilin
7	Tendon (LS)	kangaroo	H & E	20 40
8	Cartilage (ear)	human	Verhoeff's elastic, H & VG	5 10 20 20
8A	Muscle, skeletal (stretched, TS & LS)	toad	H & E	5 5 10 10 20 20 40 40
9	Bone, neonatal (tail)	rat	Alcian Blue, H & Van Gieson	5 10 10 20 20
9A	Bone (vertebrae)	kitten	Alcian Blue, H & Van Gieson	2 2 5 10 20
10	Bone (developing limb)	rat	H & E	5 5 10 10 10 20 20
10A	Bone (ground, LS)	human	Alizarin Red	5 10 20 40
11	Bone, adult (TS femur)	cat	H & E	10 20 40
11A	Bone, adult			5 10 20 40
12	Bone, developing (LS femur)	cat	H & E
12A	Bone (knee joint)	kitten	Alcian Blue, H & Van Gieson	2 2 5 5 5 10
13	Adipose tissue, white & brown	rat	H & Van Gieson	5 10 20 40
	(subscapular), muscle spindles
14	Muscle, cardiac (left ventricle)	human	H & E	5 20 40
15	Muscle, cardiac (IV septum)	sheep	PTAH	5 10 20 20 40
16	Nerve, peripheral (LS)	cat	Osmium tetroxide	5 10 20
16A	Nerve, peripheral (TS)	cat	Osmium tetroxide	2 5 20 20
17	Muscle, striated (tongue)	monkey	PTAH	5 10 20 40
18	Muscle, cardiac	rat		10 20
19	Cerebellum	cat	Luxol Fast Blue, H & E
20	Spinal cord	ox	Luxol Fast Blue, H & E	2 2 2
20A	Spinal cord	cat	Gallocyanin	2 5 5 5 20 20 40
21	Ganglion, trigeminal (sensory)	cat	H & E
21A	Ganglion, trigeminal (sensory)	cat	Luxol Fast Blue, H & E	2 10 40
22	Ganglion, sympathetic (autonomic)	human	Masson's Trichrome
23	Nerve, peripheral (sciatic)	cat	Masson's Trichrome	10 40
24	Thyroid	human	H & E
24A	Thyroid and parathyroid	chimp	Masson's Trichrome	5 10 20 40
25	Pituitary (Hypophysis)	human	Tri-PAS
26	Pituitary (Hypophysis)	ox	Picro-Mallory
27	Cerebellum	rat	Nonidez silver impregnation
28	Cerebrum	human	GFAP
28A	Cerebellum	human	GFAP
29	Adrenal	monkey	H & E
30	Adrenal	human	Masson's Trichrome
31	Neurovascular bundle (TS)	monkey	Verhoeff's Elastic, H & VG	5 10 20 40
31A	Neurovascular bundle (TS)	monkey	Toluidine Blue (resin embedded)	2 5 10 20 20
32	Vena cava	human	Verhoeff's Elastic, H & VG
33	Aorta (14 yr., TS)	human	Verhoeff's Elastic, H & VG
34	Aorta (14 yr., LS)	human	H & E
34A	Aorta (14 yr., LS)	human	Masson's Trichrome
35	Bone marrow	human	Romanowsky
36	Tonsil (palatine)	human	H & E
37	Lymph node	cat	H & E
38	Lymph node (bronchial, showing
	phagocytosed carbon)	human	H & E
38A	Lymph node (bronchial, showing	human	Hughesden's Biebrich Scarlet,
	phagocytosed carbon)		Orange G, Aniline Blue
39	Thymus (juvenile)	human	H & E
39A	Thymus (juvenile)	human	H & Van Gieson
40	Thymus (adult)	human	H & Van Gieson
40A	Thymus (neonatal)	human	H & E
41	Spleen	human	H & E
41A	Spleen	human	H & Van Gieson
42	Skin (thick), palm	human	H & E	5 10 20 20
43	Skin (thick), palm	human	Verhoeff's Elastic, H & VG	5 10 40
44	Eyelid	human	Aldehyde Pararosanilin,
			Masson's Trichrome
45	Blood (adult)	human	Romanowsky
45A	Blood (adult - leucocytosis)	human	Romanowsky
46	Lip	human	H & E
47	Skin (thin), scalp (LS and TS)	human	Gomori Trichrome, Picric Acid	2 10 10 20 20 20 40 40 40
48	Skin (thin), axillary	human	Masson's Trichrome	5 10 20 20 40
48A	Skin (thick) palmar	human	PTAH	5 10 20
49	Digit, neonatal (LS)	human	H & Van Gieson
50	Palate (soft)	dog	H & E
50A	Palate (soft)	dog	PAS, Tartrazine, Iron H.	5 10 20 40
51	Larynx (neonatal)	human	H & E, Alcian Blue
52	Tongue (filiform papillae)	human	H & E
52A	Tongue, root	human	Alcian Blue, H & Van Gieson
53	Tongue (fungiform & circumvallate)	human	Masson's Trichrome
53A	Tongue (taste buds)	rabbit	Masson's Trichrome
54	Head (neonatal)	rat	Alcian Blue, H & E	2 2 2 5 5 5 10 20 40
54A	Rat (LS)	rat	Alcian Blue, H & Van Gieson	2 5 5 20
55	Nasal cavity	monkey	Alcian Blue, H & Van Gieson
56	Head (neonatal)	rat	H & E
56A	Head (neonatal)	rat	H & Van Gieson	2 5 5 10 20 20 40
57	Cochlea	guinea pig	H & Phloxine (resin embedded)
58	Eye (anterior segment)	monkey	H & Phloxine (resin embedded)
59	Eye (posterior segment)	primate	Masson's Trichrome
60	Tooth, adult	rat	H & E
61	Salivary gland (submandibular)	human	H & E	2 2 10 10 20 20 40
62	Salivary gland (parotid)	human	H & E	20 40
63	Oesophagus (middle 1/3, TS & LS)	human	H & E
64	Oesophagus (middle 1/3, TS & LS)	human	Masson’s Trichrome	5 10 20 40
65	Cardio-oesophageal junction	primate	H & E
66	Stomach, body	chimp.	H & E
66A	Stomach, body	chimp.	PAS, H & E
67	Stomach, pylorus	primate	H & E
68	Pyloroduodenal junction	primate	H & E
69	Duodenum	human	H & E (resin embedded)
69A	Duodenum	primate	H & E
70	Intestine, small (jejunum)	chimp.	H & E	5 10 20 40
70A	Intestine, small (ileum, TS)	cat	Alcian Blue, H & Van Gieson
71	Appendix, vermiform	human	Alcian Blue, H & E	5
72	Intestine, large	human	H & E
73	Rectum	human	H & E
74	Liver	human	H & E
74A	Liver	monkey	PTAH
75	Gall bladder	human	H & E	2 5 10 10 10 20 20 20 40 40
76	Liver	pig	H & Van Gieson
76A	Liver (reticular fibres)	rabbit/monkey	Silver impregnation	5 20
77	Pancreas	human	H & E
77A	Pancreas	monkey	Phloxine, H & Tartrazine
78	Trachea	human	H & E	5 10 20 20 40 40
79	Lung	cat	Picro-Mallory	20 40
79A	Lung	human	H & E
80	Kidney (LS & TS)	rat	H & E
80A	Kidney (LS & TS)	rat	PAS, H & Tartrazine
81	Kidney (LS)	human	H & E
82	Kidney (vascular injection)	cat	Iron H, Fast Green,
			Carmine-gelatine
83	Ureter	human	H & E
83A	Ureter	chimp.	H & Van Gieson
84	Bladder, urinary (relaxed)	human	Masson’s Trichrome	5 10 20 40 40
85	Bladder, urinary (distended/relaxed)	monkey	H & E	10 20 40
86	Testis (LS)	cat	Masson's Trichrome
86A	Testis	cat	Gomori's Trichrome
87	Testis	human	H & E
87A	Testis, Epididymis	monkey	H & E
88	Vas deferens	human	H & E
89	Seminal vesicle	ox	Heidenhain's Azan & Masson's Trichrome	10 20 40
90	Prostate	chimp.	H & E
90A	Prostate	dog	H & Van Gieson
91	Penis, neonatal (TS)	human	Masson's Trichrome
91A	Spermatozoa	human	Iron Haematoxylin
92	Ovary	cat (kitten)	H & E
92A	Ovary, corpus luteum	human	H & E
93	Uterine tube (oviduct)	human	H & E
94	Cervix (LS)	human	H & E
94A	Cervix (LS)	human	PAS, Haematoxylin
95	Endometrium (secretory)	human	H & E
95A	Endometrium (late proliferative)	human	H & E
96	Uterus, Placenta	human	H & E
97	Vagina	human	H & E
97A	Vagina (lateral wall smear)	human	Shorr (modified)
98	Mammary gland (immature)	human	Heidenhain's Azan
99	Mammary gland (lactating)	cat	H & E
100	Mammary gland (post-menopausal)	human	H & E

Magnification Scale Bars

The links below are to the magnification scale bars used to calibrate the above images.

2 5 10 20 40

Histology Staining
stain nucleus  cytoplasm  collagen  RBCs  other  Haematoxylin blue       mucins - light blue  Eosin   pink  pale pink  bright red colloid - pink muscle - red  Iron Haematoxylin   blue/black              Van Gieson   brown/yellow red  yellow muscle: yellow/brown cartilage - pink Verhoeff's Elastin  black        elastic fibres - black Tartrazine   yellow yellow yellow   Silver Impregnation      grey/ brown    reticular fibres - black Methyl Green  light green  light green  light green  green   Nuclear Fast Red red pink pink pink    Gomori's Trichrome purple/red purple  green red keratin - red muscle- purple/red Heidenhain's Azan red purple/red  deep blue red muscle - red Osmium tetroxide       brown  brown myelin, lipids - black Alcian Blue         mucins - blue Periodic acid & Schiff's reagent (PAS)       pink    mucins, glycogen, glycocalyx - magenta PTAH blue   red blue  muscle bands - blue Masson's Trichrome blue /black red  green /blue red cartilage, mucins - blue or green; muscle - red  Luxol Fast Blue        variable  myelin - blue  Aldehyde Fuchsin         elastic fibres, mast cells- deep purple  Light Green      light green      Gallocyanin  dark blue       nucleic acids, Nissl granules - dark blue Romanowsky (e.g. Leishman's stain) blue pink acidophils - red basophils - blue azurophilic - purple  Aldehyde Pararosanilin          elastic fibres - purple
Haematoxylin/ Eosin	nucleus- blue  cytoplasm- red/pink
Van Gieson,  Masson's Trichrome	stain is a combination of two or more dyes.
Van Gieson	used with Iron Haematoxylin, giving brown nuclei.
Alcian Blue with Van Gieson	mucins stain green
PTAH	Phosphotungstic acid and Haematoxylin.
Luxol Fast Blue with Tartrazine	myelin may be green
Picro-Mallory	similar to Masson's Trichrome, giving orange/yellow RBCs.
	(modified from B. Freeman, 2000)

WWW Links

• Online Historic Microscopes
  • Overview of collection
• For specific systems see the Local listing by system of the WWW sites listed below.
• Loyola University Medical Education Network (LUMEN- Histology)
  • Part 2: Stains, Cells, and Ultrastructure (EM)
• University of WA- Department of Anatomy and Human Biology (Histology Notes)
• University of Kansas Department of Anatomy and Cell Biology (JayDoc HistoWeb)
• Harvard Medical School (MedWeb)
• The College of Medicine at the University of Florida (Histology Tutorial)
• Histology Glossary

• Molecular Probes
  • Organelle Probes: Golgi Apparatus and Endoplasmic Reticulum
  • Organelle Probes: Lysosomes, Vacuoles and Other Acidic Organelles
  • Organelle Probes: Mitochondria
  • Tracers: CellTracker Probes
  • Actin and Tubulin Probes
  • Bacteria Stains
  • About Flourescent Dyes and their spectra
• Bio-Rad Microscopy Products
• Leica microscopes
• Nikon microscopes
• Olympus microscopes
• Optiscan &emdash; personal confocal laser scanning microscope system
• Zeiss microscopes

About Notes

• These lecture notes from the Embryology Program compiled and written by Dr Mark Hill.

Links

Serial Sections Homepage

Human Homepage

Pig Homepage

m.hill@unsw.edu.au
Date Last Modified: 04/08/2003
This site maintained by Dr M. Hill